Molekul: Jurnal Ilmiah Kimia
Vol 19 No 1 (2024)

Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae

Sulistiyawati, Indah (Unknown)
Wahyono, Daniel Joko (Unknown)
Siswandari, Wahyu (Unknown)

Article Info

Publish Date
14 Mar 2024

Abstract

Porin messenger RNA (mRNA) expression significantly influences porin permeability and reduces antibiotic penetration. Klebsiella pneumoniae has developed resistance to several antibiotics. Decreased expression levels of OmpK35 and OmpK36 porins result in changes in the porin profile and even functional loss of porins. This research aimed to analyze the relative expression of the outer membrane porins, OmpK35 and OmpK36. The research methodology involved MIC assays and quantification of OmpK35 and OmpK36 mRNA using RT-qPCR. The relative expression of OmpK35 and OmpK36 in clinical isolate K. pneumoniae is 0.8925 and 0.5877, respectively. The porin permeability of OmpK35/K36 mRNA positively correlated with the MIC values of OmpK35 (p-value = 0.029) and OmpK36 (p-value = 0.016), respectively. Keywords: Ciprofloxacin, Klebsiella pneumoniae, OmpK35/K36, porin permeability

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Journal Info
Molekul: Jurnal Ilmiah Kimia
Website

Abbrev

jm

Publisher

Universitas Jenderal Soedirman

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

Description

The MOLEKUL is dedicated to fostering advancements in all branches of chemistry and its diverse sub-disciplines. It aims to publish high-quality research encompassing a wide range of topics, including but not limited to Pharmaceutical Chemistry, Biological Activities of Synthetic Drugs, ...