Indonesian Journal of Agricultural Research
Vol. 7 No. 1 (2024): InJAR, Vol. 7, No. 1, March 2024

Nitrogen-Fixing Purple Nonsulfur Bacteria Originating from Acid Saline Soils of a Rice-Shrimp Farm

Anh, Nguyen Hoang (Unknown)
Xuan, Ly Ngoc Thanh (Unknown)
Xuan, Do Thi (Unknown)
Quang, Le Thanh (Unknown)
Khuong, Nguyen Quoc (Unknown)



Article Info

Publish Date
01 Mar 2024

Abstract

The study was conducted to (i) isolate, select, and identify strains of purple nonsulfur bacteria (PNSB), which can fix nitrogen (N), from soil and water in a rice-shrimp integrated system, (ii) to determine the capacity of the selected potent PNSB strains in producing plant growth promoting substances. The isolation resulted in 57 pure PNSB strains from 36 soil samples and 36 water samples of rice-shrimp paddy fields in Thanh Phu - Ben Tre. Among them, 49 strains survived under pH 5.0 conditions, 24 of which grew well under microaerobic light (ML) and aerobic dark (AD) conditions in a basic isolation medium (BIM) containing NaCl 5‰. Two strains (S01 and S06) with the greatest N fixation were identified by 16S rRNA techniques as Rhodobacter sphaeroides. Their N production was 16.9 mg L-1 under the ML condition and 32.1 mg L-1 under the AD condition. Moreover, two R. sphaeroides S01 and S06 strains performed P solubilization at 0.382-2.954 mg L-1 from Al-P, 3.81-4.28 mg L-1 from Fe-P, and 3.87-4.74 mg L-1 from Ca-P, and production of plant growth promoting substances, such as IAA (12.3-15.5 mg L-1), EPS (1.09-1.58 mg L-1), siderophores (10.7-53.6%) and ALA (1.68-2.82 mg L-1) under both the incubating conditions.

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Journal Info

Abbrev

InJAR

Publisher

Subject

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Environmental Science Social Sciences Veterinary

Description

Indonesian Journal of Agricultural Research (InJAR) is a peer-reviewed quarterly journal published by TALENTA (Universitas Sumatera Utara's Journals Publisher) and managed by Faculty of Agriculture, Universitas Sumatera Utara. It is based on DIKTI accreditation standard and covers all aspects of ...