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All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA SAINS TANAH - Journal of Soil Science and Agroclimatology Indonesian Journal of Agricultural Research
Khuong, Nguyen Quoc
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Education Environmental Science Social Sciences Veterinary

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Nitrogen-Fixing Purple Nonsulfur Bacteria Originating from Acid Saline Soils of a Rice-Shrimp Farm Anh, Nguyen Hoang; Xuan, Ly Ngoc Thanh; Xuan, Do Thi; Quang, Le Thanh; Khuong, Nguyen Quoc
Indonesian Journal of Agricultural Research Vol. 7 No. 1 (2024): InJAR, Vol. 7, No. 1, March 2024
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/injar.v7i1.14726

Abstract

The study was conducted to (i) isolate, select, and identify strains of purple nonsulfur bacteria (PNSB), which can fix nitrogen (N), from soil and water in a rice-shrimp integrated system, (ii) to determine the capacity of the selected potent PNSB strains in producing plant growth promoting substances. The isolation resulted in 57 pure PNSB strains from 36 soil samples and 36 water samples of rice-shrimp paddy fields in Thanh Phu - Ben Tre. Among them, 49 strains survived under pH 5.0 conditions, 24 of which grew well under microaerobic light (ML) and aerobic dark (AD) conditions in a basic isolation medium (BIM) containing NaCl 5‰. Two strains (S01 and S06) with the greatest N fixation were identified by 16S rRNA techniques as Rhodobacter sphaeroides. Their N production was 16.9 mg L-1 under the ML condition and 32.1 mg L-1 under the AD condition. Moreover, two R. sphaeroides S01 and S06 strains performed P solubilization at 0.382-2.954 mg L-1 from Al-P, 3.81-4.28 mg L-1 from Fe-P, and 3.87-4.74 mg L-1 from Ca-P, and production of plant growth promoting substances, such as IAA (12.3-15.5 mg L-1), EPS (1.09-1.58 mg L-1), siderophores (10.7-53.6%) and ALA (1.68-2.82 mg L-1) under both the incubating conditions.
Isolating, characterizing, and utilizing Trichoderma asperellum to antagonize Neurospora spp. causing scab disease on King mandarin fruits (Citrus sinensis) Khuong, Nguyen Quoc; Ngoc, Le Yen; Thuan, Vo Minh; Hiep, Phan Chan; Trong, Nguyen Duc; Quang, Le Thanh; Thu, Le Thi My; Huy, Nguyen Gia; Nhan, Tran Chi; Xuan, Ly Ngoc Thanh; Xuan, Do Thi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.125141-157

Abstract

The research aimed to (i) isolate, select, and assess the pathogenic potential of fungi that pose a risk of causing scab disease on King mandarin fruits (Citrus sinensis), and (ii) evaluate the antagonistic potential of Trichoderma spp. against Neurospora spp., , which cause scab disease on King mandarin fruits under in vitro conditions. The isolation process identified four fungal strains from ten King mandarin fruits showing scab symptoms, collected from ten orchards in Vung Liem District, Vinh Long Province. The three most virulent scab-causing fungal strains were KMS-01, KMS-02, and KMS-04, with growth diameters of 7.60–7.63 cm after 72 hours of culture (HoC). Additionally, the antagonistic ability of 50 Trichoderma spp. strains against Neurospora spp. ranged from 49.8% to 87.6% at 72 HoC. Among these, three Trichoderma spp. strains—T-SP03, T-SP26, and T-SP41—exhibited high antagonistic efficiency (86.1%–87.7%) against all three scab-causing strains. Based on the ITS region, the pathogenic fungal strains were identified as Neurospora intermedia KMS-01, N. intermedia KMS-02, and N. crassa KMS-04, while the Trichoderma spp. strains were identified as Trichoderma asperellum T-SP03, T-SP26, and T-SP41 with 100% similarity. T. asperellum shows potential as a biological control agent against Neurospora spp., the causative agents of scab disease in King mandarin.
Restoring subsoil degradation with mixed fertilizer-conditioner: A case study on red chili pepper (Capsicum annuum) cultivation Sari, Stefina Liana; Khuong, Nguyen Quoc; Sofyan, Emma Trinurani; Rohman, Saefur; Budiarto, Rahmat; Solihin, Eso
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 22, No 2 (2025): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v22i2.97637

Abstract

The loss of topsoil in high-rainfall regions significantly reduces agricultural productivity, especially in degraded soils. This study investigated the effects of Mixed Fertilizer-Conditioner (MFC) on improving the chemical properties of subsoil cultivated with red chili peppers. A Randomized Block Design (RBD) with 11 treatments on subsoil and one control on normal soil was implemented, with three replications. The treatments included: A= subsoil without fertilizer, B= 0% MFC + full NPK, C= 25% MFC + full NPK, D= 50% MFC + full NPK, E= 75% MFC + full NPK, F= 100% MFC + full NPK, G= 50% MFC + 75% NPK, H= 50% MFC + 50% NPK, I= 50% MFC + 25% NPK, J= 50% MFC without NPK, and K= Full NPK on normal soil. The application of 100% MFC combined with full NPK significantly enhanced subsoil chemical properties. Soil organic carbon increased to 1.32%, pH rose to 6.3, CEC reached 22.1 cmol kg⁻¹, and base saturation improved to 49.4%. Nutrient availability also increased, including total N (1.21%), P (0.132%), K (0.677 cmol kg⁻¹), along with Ca (1362.72 ppm), Mg (311.04 ppm), and S (36.01 ppm). Micronutrients B, Co, and Zn also rose to 4.41 ppm, 18.95 ppm, and 11.97 ppm, respectively. Chili yields in subsoil treated with 50–100% MFC and full NPK exceeded 10 tons ha⁻¹. These results highlight the agronomic potential of MFC for rehabilitating degraded soils and recommend its use as a sustainable strategy to enhance soil fertility in low-fertility or erosion-prone areas, with implications for both farmers and agricultural policymakers.
Co-Authors Anh, Nguyen Hoang Emma Trinurani Sofyan Eso Solihin Hiep, Phan Chan Huy, Nguyen Gia Ngoc, Le Yen Nhan, Tran Chi Quang, Le Thanh Rahmat Budiarto Rohman, Saefur Sari, Stefina Liana Thu, Le Thi My Thuan, Vo Minh Trong, Nguyen Duc Xuan, Do Thi Xuan, Ly Ngoc Thanh