The International Science of Health Journal
Vol. 2 No. 4 (2024): December: The International Science of Health Journal

Identification Of Metallo?-Lactamses and Integron Genes In Pseudomonas Aeruginosa Isolated From Burn Injury Patients: Phenotype And Genotype

Ashraf Ayyal Mutar Alrashedi (Unknown)



Article Info

Publish Date
04 Oct 2024

Abstract

Pseudomonas aeruginosa, which generates metallo-?-lactamase (MBL), is the cause of infections linked to burn patients, and this is a growing global problem. The objectives of the  recent study were  to determine antibiotic susceptibility of P. aeruginosa isolates,  the presence of  MBLs genes, and Integron gene class-1 . One hundred burn patients at the Al-Hussein Teaching Hospital in Al Smawah, Iraq, were isolated clinically. Twenty (20%) of these were determined to be P. aeruginosa by 16sRNA and biochemical testing. To further identify the antibiotic sensitivity for each isolate was employed by Disc Diffusion Method . The phenotypic MBL production For detected isolates was evaluated by Combined Disc Diffusion Method (CDD). Integron gene class -1 (Int-1) and Genes  encoding  MBLs were identified by Polymerase Chain Reaction (PCR). P. aeruginosa was shown to be totally resistant to ceftriaxone, ampicillin, piperacillin, gentamicin, and ciprofloxacin in this investigation. 90% of the isolates were determined to be multidrug resistant, and different levels of moderate to lowest resistance were observed in Amikacin, Meropenem, Aztreonam, Cefixime, and Levofloxacin. While 90% of the isolates possessed the Int-1 gene, our recorded the absence of  All MBLs genes (Bla IMP, Bla VIM,  Bla GIM ) From All isolates under investigation. Illustrating for the first time how crucial it is to put in place appropriate infection control measures in order to give patients the best care possible and stop the development of these resistant microbes among burn patients.

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Journal Info

Abbrev

ishel

Publisher

Subject

Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

Description

Praktek Ilmu Bedah, Farmasi, Persalinan, Kesehatan Anak, Kesehatan Jiwa, Kesehatan Masyarakat, Kedokteran Gigi, Ilmu Pendidikan Kesehatan, Kebidanan, Ilmu Kesehatan dan Keperawatan, Kebijakan dan Manajemen Kesehatan, Gizi Masyarakat, Kesehatan Lingkungan, Epidemiologi, Kesehatan Reproduksi, Promosi ...