Alchemy : Journal of Chemistry
Vol 11, No 2 (2023): ALCHEMY: JOURNAL OF CHEMISTRY

Antioxidant Activity of Mahogany Parasite Leaves (Dendrophthu pentandra (L.) Miq.) Assessed Using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) Method

Reza, Muhammad (Unknown)
Nurdin, Nurmalahayati (Unknown)
Salsabila B.S., Siti Raihan (Unknown)



Article Info

Publish Date
12 Nov 2023

Abstract

Synthetic antioxidants, such as butyl hydroxyanisole (BHA), have carcinogenic (cancer-causing) adverse effects because they can cause type I alveolar damage and damage to lung endothelial genes. The objective of this study was to use the DPPH (2,2 diphenyl-1-picrylhydrazyl) method to investigate the antioxidant activity of natural substances found in the leaves of the mahogany parasite (Dendrophthu pentandra (L.) Miq.). Extraction, chromatography, and spectrophotometry are the techniques used. Maceration in stages utilizing n-hexane, ethyl acetate, and ethanol as solvents yielded mahogany parasite leaf extract. Phytochemical screening, thin layer chromatography testing, antioxidant activity testing utilizing a Visible Spectrophotometer, and estimating the IC50 were all performed on mahogany parasite leaf extract. The phytochemical screening results revealed that the samples included four types of secondary metabolites: flavonoids, tannins, saponins, and steroids. A thin layer chromatographic test revealed four well-separated stains using n-hexane and ethyl acetate eluent in a volume ratio of 5:5, showing the Rf as the value of the four secondary metabolites mentioned above. Based on the results of the experiments, it is possible to conclude that mahogany parasite leaves have strong antioxidant activity, with an IC50 of 8.47 ppm.

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Journal Info

Abbrev

Kimia

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Materials Science & Nanotechnology

Description

ALCHEMY: Journal of Chemistry (eISSN 2460-6871) is a scientific journal that focus on chemistry. This journal publishes a scientific article that cover research and review articles. Research topics for this journal such as natural science, physical chemistry, inorganic chemistry, organic chemistry, ...