<![CDATA[This study investigates the effect of microbial configuration on the electrochemical performance of photosynthetic microbial fuel cells (PMFCs). The PMFC configuration incorporating both bacteria and microalgae exhibited the highest open-circuit voltage (OCV) of 397.95 ± 31.53 mV, significantly higher than that of the OCVs obtained in the sterile control (C1) and the microalgae-only configuration (C2), which were 32.47 ± 22.43 mV and 284.59 ± 12.63 mV, respectively. Furthermore, the PMFC containing only microalgae achieved a current density (CD) of 20.96 ± 0.18 mA/m³ and a power density (PD) of 0.40 ± 0.01 mW/m³ under room temperature conditions. Notably, the combined bacteria and microalgae configuration demonstrated a substantial performance improvement, yielding a significantly higher CD of 49.33 ± 0.36 mA/m³ and PD of 0.78 ± 0.01 mW/m³ at room temperature. This configuration also achieved a maximum decolorization of 93.57 ± 0.10% with a corresponding algal biomass recovery of 134.90 ± 2.69 mg/L. These findings highlighted the critical role of microbial composition in PMFC performance. The combination of bacteria and microalgae yielded superior results compared to other configurations under the investigated conditions.]]>
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