<![CDATA[Fish seed is a primary factor in the success of aquaculture activities. However, aquaculture farmers are finding it difficult to obtain quality seed suppliers, leading to a lack of supply in some areas. This causes farmers to rack their brains. One way that can be done is by conducting a closed-system of wet transportation from a quality seed supplier. Chemical and natural anesthetics can be used to keep fish seeds alive in a healthy condition and minimize mortality during transportation. Chemical synthetic anesthetics have disadvantages if used continuously because they can reduce immune function and interfere with the health of the seed. Therefore, a literature review was conducted to provide information on a variety of natural compounds that can be used as a substitute for synthetic chemicals in anesthetizing fish seed during the closed-system of wet transportation using the literature study method. Natural ingredients used as anesthetics to replace synthetic chemicals are clove oil, citronella oil, tuba root extract, cassava extract of inner accession, papaya leaf extract, keben fruit, bandotan leaf extract, and guava leaf extract. Based on these natural anesthetic ingredients, the tree most effective types were identified. Cassava extract of inner accession yielded the most optimal results for transporting tilapia seeds with a survival rate of 100%. Additionally, papaya leaf extract provided the best results for tiger grouper seeds survival at 100%, while tube root extract showed a 98.3% survival rate for tilapia seeds.]]>
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