<![CDATA[Biobutanol is an example of altemative energy sources to replace 1 i qui d fuel with the carbon-neutralcharacteristic. It has more benefits to the environment compared to the fossil fuel. Biobutanol is synthesizedthrough fermentation of microalgae cells wali or other organism parts as the carbon sources. The aim of thisstudy is to determine the ability of Clostridium acetobutylicum bacteria in the fermentation of Nannochloropsissp. to produce biobutanol. Fermentation of Nannochloropsis sp. for biobutanol production was used as an initialtreatment before lipid extraction. Fermentation was performed with C. acetobutylicum bacteria for 96 hours. Theresult showed that C. acetobutylicum was able to produce 2.61% v/v butanol. This process used Nannochloropsissp. microalgae hydrolysates and biomass of viscozyme hydrolysis yield. The process of hydrolysis with celluloseand viscozyme can produce simple sugars, with the highest obtained yield of 1738.38 ppm from hydrolysis usingviscozyme.]]>
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