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All Journal Microbiology Indonesia LEMBARAN PUBLIKASI MINYAK DAN GAS BUMI Scientific Contributions Oil and Gas
ONIE KRISTIAWAN
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology

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Construction and Expression of Single Recombinant Peptide Surfactant for EOR Application CUT NANDA SARI; USMAN USMAN; RIESA KW ROHMAT; LENI HERLINA; KEN SAWITRI SULIANDRI; ONIE KRISTIAWAN; DWIYANTARI DWIYANTARI; TATI KRISTIANTI; SONY SUHANDONO
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1420.163 KB) | DOI: 10.5454/mi.11.1.5

Abstract

Surfactant is generally synthetic chemical, which is effective and reliable. However, the chemicals usually did not degraded easily in the environment and could cause damage to the environment. The other possible alternative to produce surfactant is using genetic engineering in order to produce peptide based surfactant. In this research, peptide surfactant was produced using a gene construct which was created using overlapped polymerase chain reaction method (OE-PCR). PAGE analysis shows that single surfactant peptide construction can be expressed by induction of IPTG 1 mM and after at least twice sonication. This research proves that both two constructions have been successfully expressed by producing peptide in expected size (approximately 15 kDa).
BIODEGRADASI SENYAWA HIDROKARBON MINYAK BUMI MENGGUNAKAN AKTIFITAS BAKTERI LAUT DALAM Syafrizal; Restiya Rahmaniar; Tri Partono; Zulkifliani; Onie Kristiawan; Novie ArdhyArini; Yanny Handayani; Rofiqoh
Lembaran Publikasi Minyak dan Gas Bumi Vol. 54 No. 2 (2020): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Pencemaran minyak bumi semakin banyak terjadi dengan semakin meningkatnya permintaan minyak untuk dunia industri, meningkatnya jumlah anjungan pengeboran minyak lepas pantai, dan meningkatnya transportasi laut.Beberapa cara penanggulangan tumpahan minyak meliputi penanggulangan mekanis, pembakaran in situ, kimiawi, dan bioremediasi. Bioremediasi adalah penggunaan makhluk hidup, khususnya mikroorganisme untuk mendegradasi atau mendetoksifikasi pencemar lingkungan. Mikroorganisme yang digunakan dapat berupa bakteri alami yang berasal dari daerah yang tercemar maupun bakteri yang diisolasi dari daerah lain lalu diintroduksi ke daerah yang tercemar.Penelitian ini bertujuan menguji kemampuan bakteri laut dalam mendegradasi minyak menggunakan media yang berbeda, yaitu antara media seawater nutrient broth dan media air terformasi, serta menyimulasikan proses degradasi minyak yang mengalami dispersi dalam sebuah microcosmPenelitian ini merupakan peningkatan skala dari penelitian Dwinovantyo (2015) dan modifikasi dari penelitian Cappello et al. (2006) dan Darmayati et al (2015). Simulasi degradasi tumpahan minyak dilakukan menggunakan media bervolume 8 liter berisi air laut yang tercemar tumpahan minyak, kemudian dilakukan pengamatan populasi bakteri serta kandungan tumpahan minyak. Bakteri yang digunakan adalah konsorsium dari Raoultella sp., Pseudomonas sp., dan Enterobacter sp. yang berasal dari sedimen laut dalam hasil isolasi dan identifikasi Dwinovantyo (2015).
PENGAMATAN MEOR MENGGUNAKAN FORMULA NUTRISI RENDAH GLUKOSA DENGAN METODA IMBIBISI Sugihardjo; Zulkifliani; Onie Kristiawan; Cut Nanda Sari; Syafrizal
Lembaran Publikasi Minyak dan Gas Bumi Vol. 53 No. 1 (2019): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Kegiatan skala laboratorium telah dilakukan untuk merancang formula nutrisi MEOR (Microbial Enhanced Oil Recovery) dengan bahan dasar rendah-glukosa. Bahan yang dipakai meliputi: limbah cair tahu, air kelapa, limbah cair ikan, limbah pengolahan pati, ekstrak teh, pupuk cair, ekstrak daging sapi, dan pepton. Bahan-bahan tersebut diracik untuk menjadi beberapa formula yang dapat merangsang pertumbuhan mikroba yang terkandung pada percontoh fluida dari sumuran SMR-01, SMR-02, dan SMR-03 sehingga menghasilkan bioproduk yang diperlukan untuk MEOR Pada seleksi kajian awal ada 48 formula nutrisi, kemudian diseleksi dan diperoleh yang potensial dalam pertumbuhan mikroba sebanyak 8 formula, yaitu 2 macam formula untuk SMR-01, 3 untuk SMR-02, dan 3 juga untuk SMR-03. 8 formula tersebut diinkubasi selama 7 hari dan diamati bioproduknya yang meliputi: pertumbuhan mikroba, pH, IFT, densitas, dan viskositas minyak. Pada tiap formula juga ditambahkan konsorsium mikroba exogenous untuk memperkaya jenis mikroba. Uji imbibisi dilakukan terhadap 8 formula tersebut serta ditambah 8 formula lagi dengan menambahkan konsorsium mikroba exogenous selama 71 hari. Hasil yang terbaik ternyata ada pada formula Ef1 dengan penambahan ekstrak teh dan pepton dan Ez1 dengan tambahan ekstrak teh dan ekstrak beef pada fluida SMR-02. Dengan nilai masing masing nilai RF (recovery factor) 56,91 untuk Ef1 dan 55,86% untuk Ez1. Oleh karena itu kedua formula tersebut dapat dijadikan acuan untuk implementasi lapangan. Secara ekonomis mungkin Ez1 akan lebih murah karena prosentase kandungan extract beef hanya 10%.
PENGARUH BIKARBONAT TERHADAP PERTUMBUHAN MIKROALGA NANNOCHLOROPSIS SP. SEBAGAI SUMBER BIOMASSA BIOFUEL Onie Kristiawan; Zenitha Lintang Agustin; Dhiti Aliya Hanupurti; Rino Nirwawan; Dian Hendrayanti
Lembaran Publikasi Minyak dan Gas Bumi Vol. 52 No. 2 (2018): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Mikroalga merupakan alga kecil (ukuran 2-20 μm) berupa tanaman talus yang memiliki klorofil sehingga mampu melakukan fotosintesis. Salah satu jenis mikroalga yang berpotensi untuk diambil minyaknya adalah Nannochloropsis Nannochloropsis sp. merupakan sel berwarna kehijauan, tidak motil, dan tidak berflagela. Sebagian besar mikroalga menggunakan cahaya dan karbondioksida (CO2 ) sebagai sumber energi dan sumber karbon. Pada perairan CO2 terlarut biasanya dalam bentuk bikarbonat (HCO3 - ). Tujuan penelitian ini untuk mengetahui pengaruh peningkatan konsentrasi bikarbonat (HCO3 - ) terhadap peningkatan pertumbuhan mikroalga Nannochloropsis sp.
THE INFLUENCE OF MEDIA COMPOSITION TO THE GROWTH OF SCENEDESMUS SP. MICROALGAE IN VARIED MEDIA Mochammad Fierdaus; Onie Kristiawan
Scientific Contributions Oil and Gas Vol 38 No 3 (2015)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.38.3.550

Abstract

Media culture is one of the important factors in the growth of microalgae. Culture medium contains macro and micro nutrition required for the growth of microalgae. Composition of nutrition and nutrition concentrations determine the production of microalgae biomass and nutrient content. The aim of this study is to evaluate the effect of medium composition variation to Scenedesmus sp growth in laboratory-scale in order to get the optimized composition of media for microalgae Scenedesmus sp. This study was conducted in a laboratory scale. The growth medium that were used in this study were: BBL sm, SS, MN, M4N, “Sederhana 2”, and “Sederhana 3” medium. The microalgae that were used in this study were mixed culture Chlorophyceae microalgae which is dominated by Scenedesmus sp. The parameters observed were pH, optical density (OD), cell density and temperature. The results show that of the 6 variations of the growth medium used in this study M4N medium is the best medium for the growth of microalgae. At the end of the operation, cell density of Scenedesmus sp. from M4N medium was 306.83 x 106 cell/mL with a dark green color for the culture. The coefficient growth of Scenedesmus sp. which is grown in M4N medium was 0,36. This is likely to be due to the composition of nutrients in the medium M4N which were more complete than other media.
MICROALGAE BIOMASS PRODUCTIVITY BY CO2 INJECTION IN CORRELATION WITH pH VARIATION IN PHOTOBIOREACTOR Onie Kristiawan
Scientific Contributions Oil and Gas Vol 37 No 1 (2014)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.37.1.624

Abstract

Microalgae can bring solution for reducing CO2 and as alternative sustainable energy source. CO2reduction by microalgae was done by fi xation of CO2 during photosynthesis while they grow. The objectiveof the study is to get pH condition from CO2 injection into cultivated microalga that gives better productivityof biomass. Microalga that used was Scenedesmus.sp. Scenedesmus.sp did grown better in Walne media onlab scale experiments. CO2 injection as pH control that gave the highest dry weight obtained on pH rangevalue 7.0-7.5. As one of the controlling factor that determines the biological ability of microalgae in utilizingnutrient, pH value shows that at range of pH 8.0 – 8.5 and 9.0 – 9.5 can decrease photosynthetic process onmicroalgae Scenedesmus.sp Thus the highest fi xated CO2 also on pH value 7.0 – 7.5. This research give abrief explanation that in the range of pH 7.0 – 7.5 contain more CO2 than range pH 8.0 – 8.5 and 9.0 – 9.5.Culture temperature did not show any high fl uctuation at morning and in the mid-day of treatment.
THE EXTRACTION OF OIL FROM Nannochloropsis sp. MICROALGAE USING ULTRASONIC AND FERMENTATION AS CELL DISRUPTION Dhiti Adiya Hanupurti; Onie Kristiawan
Scientific Contributions Oil and Gas Vol 37 No 1 (2014)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.37.1.626

Abstract

Algae are a promising source of biofuel but claims about their lipid content can be ambiguous becauseextraction methods vary and lipid quantitation often does not distinguish between particular lipid classes.One of algae types that meet this condition is Nannochloropsis sp. Two different cell disruption methods,i.eultrasonic and fermentation followed by maceration extraction using a mixture of ethanol/hexane (1:1, v/v)as a solvent extraction were studied for their effectiveness in extraction of algae lipids from Nannochloropsissp. Contact time and amplitude were varied in ultrasonic process. Hydrolysis treatment was varied infermentation process, i.e Dillute Acid Pretreatment (DAP) hydrolysis, enzymatic hydrolysis and DAP withenzymatic hydrolysis. The result showed that ultrasonic process followed by maceration extraction wasmore effective for Nannochloropsis sp. lipid extraction. The most ideal treatment was at amplitude 40%and contact time 5 minutes with lipid content of 63.59% of dry weight.
Co-Authors CUT NANDA SARI Dhiti Adiya Hanupurti Dhiti Aliya Hanupurti Dian Hendrayanti DWIYANTARI DWIYANTARI Ken Sawitri Suliandari KEN SAWITRI SULIANDRI LENI HERLINA Mochammad Fierdaus Novie ArdhyArini Restiya Rahmaniar RIESA KW ROHMAT Rino Nirwawan Rofiqoh RUKMAN HERTADI SONY SUHANDONO Sugihardjo syafrizal TATI KRISTIANTI Tegar Nurwahyu Wijaya Tri Partono Usman Usman Sumo Friend Tambunan Usman Usman Yanny Handayani Zenitha Lintang Agustin Zulkifliani