This study was to determine the effect of medium thickness and fermented coconut dregs on biomass of silkworms (Tubifex sp.). This research was conducted in January - March 2021 at the Laboratory of Fish Hatchery and Breeding, Faculty of Fisheries and Marine Affairs, University of Riau. The method used in this study was two-factors Completely Randomized Design (CRD) with ten treatments and three replications. The treatments in this study were K1D40 (1cm media thickness at, 40% coconut pulp dregs dose), K1D30 (1cm media thickness at, 30% coconut pulp dregs dose), K1D20 (1cm media thickness at, 20% coconut pulp dregs dose), K1,5D40 (1.5cm media thickness at, 40% coconut pulp dregs dose) K1D30 (1.5cm media thickness 30% coconut pulp dregs dose) K1,5D20 (1.5cm media thickness 20% coconut pulp dregs dose) K2D40 (2cm medium thickness coconut pulp dregs 40% dose) K2D30 (2cm media thickness dose 30% coconut pulp dregs) K2D20 (2cm media thickness with 20% coconut pulp dregs). The best results were obtained in the K2D20 treatment (2cm media thickness, 20% coconut pulp dregs) which resulted in population growth of 4591 ind., growth of biomass of Tubifex sp. worms. K2D20 with a yield of 14.54 g, the highest length increase in the K2D20 treatment was 2.38 cm, the temperature during the study ranged from 26°C - 28°C, pH 6 – 7, dissolved oxygen (DO) ranged from 7.6 ppm – 8.4 ppm and the water discharge ranges from 0.05 to 0.1 liters/sec
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