Jurnal Biologi Tropis
Vol. 24 No. 1 (2024): Januari - Maret

Isolation and Identification of Biosurfactant-Producing Bacteria in An-aerobic Palm Oil Waste Pools at PT. Aek Loba Plantation

Sipahutar, Lara Santi Hemat (Unknown)
Rasyidah (Unknown)
Mayasari , Ulfayani (Unknown)



Article Info

Publish Date
20 Jan 2024

Abstract

Biosurfactant-producing bacteria are bacteria that have alternative energy sources, namely surfactants which contain hydrophilic and hydrophobic groups based on their molecular structure so that they can survive between different polar and hydrogen bonding fluids. Palm oil mill liquid waste is waste in the form of water or liquid, oil and organic solids produced from the processing of palm oil. The purpose of this study was to determine the genera of biosurfactant-producing bacteria found in palm oil wastewater in the an-aerobic pond of PT. Aek Loba plantation and to find out the bacterial species with the highest emulsification index. This study used descriptive methods and identification of bacteria through morphological characterization, gram staining tests and biochemical tests. Bacteria were isolated from palm oil wastewater by dilution method 10-4, 10-5 and 10-6. Bacterial emulsification index is calculated by emulsification test. In this study, 8 biosurfactant-producing bacterial isolates were found, consisting of 2 Proteus genus isolates, 2 Enterobacter genus isolates, 2 Bacillus genus bacterial isolates and 2 Pluralibacter genus isolates. The bacteria that had the highest emulsification index was Pluralibacter gergoviae with an emulsion index of 43% which was identified by molecular testing.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...