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All Journal Jurnal Biologi Tropis IJOEP : International Journal of Ecophysiology Jurnal Bioteknologi & Biosains Indonesia
Mayasari , Ulfayani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Screening of Extract Secondary Metabolites of Bacteria Which Have Symbiosis with Sponges from Central Tapanuli Bakar Island as an Antibacteria mirnawati, mirnawati; Rasyidah; Mayasari , Ulfayani; Gultom, Endang Sulistyarini
International Journal of Ecophysiology Vol. 6 No. 2 (2024): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v6i1.17821

Abstract

This study aims to determine the presence of antibacterial-producing bacteria from sponges from Bakar Island, Central Tapanuli Regency and to determine the antibacterial activity of methanol extracts from potential bacterial isolates against Staphylococcus aureus and Escherichiacoli. 21 pure isolates were found and it was known that 9 isolates produced antibacterial against S. aureus and 4 isolates against E. coli. Bacterial isolates with the highest inhibition zone against bothS.aureus and E. coli were found in isolates with code SP20 with each large inhibition zone are 14.5 mm and 16.04 mm. Then microscopic observations were carried out, namely gram staining and were obtained 8 isolates were gram positive, and 1 gram was negative. The biochemical test showed that SP20 showed positive results for the Motility, TSIA and Catalase Test but negative for Citrate Test. Bacterial isolates withthe code SP20 had the largest inhibition zone so that these isolates were to be extracted.The resultsof the screening test showed that the secondary metabolitesof the sponge symbiont bacterial extract from Bakar Island were positive for flavonoids and saponins. And then test the activity of secondary metabolites with 3 concentrations, 10%, 20%, and 30%.The results showed that the best concentration of the test bacteria was S. aureus at a concentration of 30% with an inhibition zone of 3.08 mm and E. coli at 5.04 mm.
Isolation and Identification of Biosurfactant-Producing Bacteria in An-aerobic Palm Oil Waste Pools at PT. Aek Loba Plantation Sipahutar, Lara Santi Hemat; Rasyidah; Mayasari , Ulfayani
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6423

Abstract

Biosurfactant-producing bacteria are bacteria that have alternative energy sources, namely surfactants which contain hydrophilic and hydrophobic groups based on their molecular structure so that they can survive between different polar and hydrogen bonding fluids. Palm oil mill liquid waste is waste in the form of water or liquid, oil and organic solids produced from the processing of palm oil. The purpose of this study was to determine the genera of biosurfactant-producing bacteria found in palm oil wastewater in the an-aerobic pond of PT. Aek Loba plantation and to find out the bacterial species with the highest emulsification index. This study used descriptive methods and identification of bacteria through morphological characterization, gram staining tests and biochemical tests. Bacteria were isolated from palm oil wastewater by dilution method 10-4, 10-5 and 10-6. Bacterial emulsification index is calculated by emulsification test. In this study, 8 biosurfactant-producing bacterial isolates were found, consisting of 2 Proteus genus isolates, 2 Enterobacter genus isolates, 2 Bacillus genus bacterial isolates and 2 Pluralibacter genus isolates. The bacteria that had the highest emulsification index was Pluralibacter gergoviae with an emulsion index of 43% which was identified by molecular testing.
ISOLATION OF BACTERIA AS A BIOREMEDIATION AGENT FOR RECLAMATION OF MERCURY-CONTAMINATED SOILS Idris, Muhammad; Nasution, Rizki Amelia; Mayasari , Ulfayani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1743

Abstract

Gold mining commonly involves the use of mercury, which generates hazardous and toxic mercury-based waste, leading to soil contamination and adverse effects on human health. Bioremediation has been explored as a potential solution to this issue. This study aimed to identify indigenous microbial species with potential as bioremediation agents, assess their ability to influence chemical properties, and evaluate their effectiveness in reducing mercury content. The research was conducted in five stages: isolation of native bacteria, testing the potential of microorganisms, assessing the ability to reduce mercury, conducting soil tests, and identifying bacterial characteristics through microscopic and biochemical analyses. The results revealed three types of indigenous microbes, namely Pseudomonas, Neisseria, and Klebsiella bacteria, with the highest potential as bioremediation agents. These bacterial isolates were found to enhance the availability of phosphorus in the soil, maintain soil pH, but had no effect on total soil nitrogen. Furthermore, the bacterial isolates exhibited the ability to reduce mercury content after treatment with NA isolates.
Co-Authors Gultom, Endang Sulistyarini Jayawarsa, A.A. Ketut Muhammad Idris Nasution, Rizki Amelia Rasyidah Sipahutar, Lara Santi Hemat