Jurnal Biologi Tropis
Vol. 24 No. 1 (2024): Januari - Maret

Identification of Coliform Bacteria on Ice Crystal in Langsa City

Sitorus, Siti Rahmawati (Unknown)
S. Pandia, Ekariana (Unknown)
Atmaja, Teuku Hadi Wibowo (Unknown)



Article Info

Publish Date
13 Feb 2024

Abstract

Water is the second most important element for life after air. Water is a clear liquid, odorless, colorless, and tasteless. Water is a basic need for living creatures, especially drinking water. One way of processing drinking water is by freezing it into ice. Ice cubes are a food that is widely known and is usually considered safe for consumption. Coliform bacteria serve as a general marker of contamination in food and water. Coliform bacteria found in the human body can cause several diseases, including diarrhea. The aim of this research was to confirm the presence of coliform bacteria, measure the level of contamination, and identify the specific type of coliform bacteria that contaminated crystal ice from three ice producers located in Langsa City. This research is descriptive in character. Ice crystal samples were tested using the Most Probable Number (MPN) approach. Furthermore, bacteria were identified through microscopic analysis using the gram staining method and biochemical testing, especially IMViC. Each ice crystal sample showed coliform contamination, exceeding the precautionary limits set out in Minister of Health Regulation number 492 of 2010. The highest amount of contamination was 6 MPN/100mL and the lowest amount of contamination was 4 MPN/100 mL. There were 2 types of coliform bacteria found in this study, namely: Escherichia coli and Enterobacter aeroganes.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...