Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences)
Vol. 14 No. 3 (2024): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve

Extended-spectrum beta-laktamase (ESBL) enzyme producing bacteria isolated from rectal swabs of around animals in dairy farms

Dameanti, Fidi Nur Aini Eka Puji (Unknown)
F, Jacky T. Amanda (Unknown)
Safri, Muhammad Ali A. (Unknown)
Hasan, Chyntia S. Y. (Unknown)



Article Info

Publish Date
21 Oct 2024

Abstract

This study aims to identify bacterial contamination of Klebsiella pneumoniae and Escherichia coli producing Extended Spectrum Beta Lactamase (ESBL) isolated from rectal swabs of local animals on a dairy farm in Deyeng Village, Kediri, East Java. Interviews and observations were conducted with farmers to obtain the required risk factor data (cleanliness of drinking and eating places, drinking water sources, and administration of antibiotics to livestock). The number of samples tested in this study was 34 samples, including 12 chicken samples, 8 goat samples, 5 duck samples, 4 rabbit samples, 3 cat samples and 2 goose samples. Samples were isolated on MCA media + cefotaxime 1 mg/l and isolated again on MCA media without the addition of antibiotics to multiply pure bacterial colonies. The tests carried out to determine the presence of ESBL-producing bacteria are biochemical tests, namely the IMViC test (Indole, Methyl red, Voges-Proskauer, Citrate Utilization), TSIA (Triple Sugar Iron Agar) test, gram staining, and the Double Disk Standard Method test using β-lactam disc antibiotics, namely cefotaxime 30μg, ceftazidime 30μg, and amoxycilline-clavulanate acid 30μg. The results of this research were that 6 samples or 17.6% of ESBL-producing bacteria were identified from poultry groups (chickens, ducks and geese) from dairy farms in Deyeng Village, Kediri Regency, East Java.

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Journal Info

Abbrev

JIPVET

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Veterinary

Description

Jurnal Ilmu Peternakan dan Veteriner Tropis diterbitkan oleh Fakultas Peternakan Universitas Papua. Jurnal ini merupakan media komunikasi ilmiah dibidang peternakan dan veteriner yang berupa hasil penelitian atau telaah pustaka (review) yang meliputi produksi ternak, nutrisi dan makanan ternak, ...