Comparison of Antioxidant Activity Based on Temperature Variables of Avocado Seed Extract from Maumere by 1,1-Diphenyl-2-Picrylhdrazy Method

Pratama, Mamat
Kusuma, Andi Trihadi
Rahmat, Sri Rahayu
Safitri, Dewi
Efendi, Nurmaya

Pharmaceutical Reports
Vol 3, No 2 (2024): (October) Pharmaceutical Reports

Avocados are plants that can grow well in tropical environments like Indonesia. People love this fruit because of its high antioxidant content and its delicious taste. The aim of this study was to measure the antioxidant activity of three extracts of ethyl acetate of avocado seeds (Percea americana Mill.) using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The measurement was done using a quersetin as a reference standard with a maximum wavelength of 516 nm. The results of the study showed that the sample's half inhibitory concentration (IC50) value was 1,653.89 g/mL, 1,202.56 g/ mL, and 903.08 g/mL for two, four, and six hours, respectively.