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Antioxidant Activity Test of Ethyl Acetate Extract of Avocado Seeds (Persea americana Mill.) Using the 1,1-Diphenyl-2-Picrylhdrazyl Method Rahmat, Sri Rahayu; Pratama, Mamat
Pharmaceutical Reports Vol 3, No 1 (2024): (March) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v3i1.288

Abstract

Avocado is a plant that can thrive in tropical areas such as Indonesia and is one of the types of fruit that is popular with the public, however, avocado seeds have not been used optimally so that they are wasted as waste. Based on phytochemical experiments, avocados also contain phenolics, tannins and flavanoids which have antioxidant activity. Antioxidants are compounds that can be used to counteract free radicals. Dietary fiber and antioxidants are two types of components that are very useful in improving health and being able to prevent various diseases. The extraction method used in this study is reflux where the sample is heated for 6 hours with the solvent used to evaporate at high temperatures, but it will be cooled by the condenser so that the solvent which was in the form of vapor will condense in the condenser and fall again into the reaction vessel so that the solvent will still be there during the reaction. This study aims to analyze the antioxidant activity of the ethyl acetate extract of avocado seeds using the DPPH method. Quantitatively, the ethyl acetate extract of avocado seeds (Persea americana Mill.) was measured at a maximum wavelength of 516 nm using quercetin as a reference standard. The research results showed that the value was 903.08 μ/mL. Quantitatively, the ethyl acetate extract of avocado seeds (Persea americana Mill.) was measured at a maximum wavelength of 516 nm using quercetin as a reference standard. The research results showed that the value was 903.08 μ/mL. Quantitatively, the ethyl acetate extract of avocado seeds (Persea americana Mill.) was measured at a maximum wavelength of 516 nm using quercetin as a reference standard. The research results showed that the value was 903.08 μ/mL.
Comparison of Antioxidant Activity Based on Temperature Variables of Avocado Seed Extract from Maumere by 1,1-Diphenyl-2-Picrylhdrazy Method Pratama, Mamat; Kusuma, Andi Trihadi; Rahmat, Sri Rahayu; Safitri, Dewi; Efendi, Nurmaya
Pharmaceutical Reports Vol 3, No 2 (2024): (October) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v3i2.308

Abstract

Avocados are plants that can grow well in tropical environments like Indonesia. People love this fruit because of its high antioxidant content and its delicious taste. The aim of this study was to measure the antioxidant activity of three extracts of ethyl acetate of avocado seeds (Percea americana Mill.) using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The measurement was done using a quersetin as a reference standard with a maximum wavelength of 516 nm. The results of the study showed that the sample's half inhibitory concentration (IC50) value was 1,653.89 g/mL, 1,202.56 g/ mL, and 903.08 g/mL for two, four, and six hours, respectively.