Jurnal Farmasi Sains dan Praktis
Vol 10 No 3 (September-December 2024)

ANALYSIS OF LYCOPENE CONTENT IN RED GUAVA JUICE (PSIDIUM GUAJAVA L.) BY VISIBLE SPECTROPHOTOMETRY

Hastuti, Aprilia Asri Hastuti Asri (Unknown)
Dhurhania, Crescentiana Emy (Unknown)



Article Info

Publish Date
22 Dec 2024

Abstract

Red guava (Psidium guajava L.) is a fruit that is rich in phytochemicals that are beneficial for health and is widely consumed in the form of juice. Lycopene belongs to the carotenoid group which has antioxidant potential and has an important role in the immune system to deal with homeostatic changes caused by oxidative stress. Lycopene is contained in a lot of red fruit. The purpose of this study was to analyze the lycopene content in red guava juice by visible spectrophotometry. Red guava juice extracted using n-hexane:acetone:methanol 1:2:1. Qualitative analysis was carried out by observing the spectrum, then measuring the maximum wavelength, which was supported by observing the spots by thin layer chromatography on silica gel 60 GF254 plate using petroleum ether:acetone 9:1. Quantitative analysis was carried out by measuring absorbance by visible spectrophotometry at 470 nm. The results of qualitative analysis by visible spectrophotometry obtained 3 typical peaks of the lycopene spectrum at 400-550 nm, with a typical maximum wavelength of lycopene at 470 nm. Qualitative analysis by thin layer chromatography obtained spot with Rf 0.64. The results of quantitative analysis obtained lycopene levels of 2.91 ± 0.465 mg/100 grams of juice.

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Journal Info

Abbrev

pharmacy

Publisher

Subject

Immunology & microbiology Medicine & Pharmacology Public Health

Description

Jurnal Farmasi Sains dan Praktis aims to serve the updated scientific knowledge for international researchers in pharmaceutical sciences. Jurnal Farmasi Sains dan Praktis (JFSP) publishes review article, original article, as well as short communication in the all scopes of Pharmaceutical Science ...