Euthynnus affinis is home to a variety of metazoa parasites, including monogenae, digenea, acanthocephala, and nematodes. Anisakis sp. is a parasitic nematode that causes host-related diseases. The purpose of this study was to determine the prevalence and degree of parasite infection by Anisakis sp. in stalk fish landed at the fish auction site (FAS) of the City of Oeba-Kupang. The research used a comparative descriptive method. Laboratory tests used for the identification and prevalence of the parasite Anisakis sp. refer to SNI 2332.6:2015. The results of the study showed that the Anisakis sp. parasite was most commonly found in the intestines of barley fish (21), liver (17), stomach (13), and gonads (seven). Calculation of the prevalence of eight positive specimens infected with the Anisakis sp. parasite showed a prevalence of 20%, which belongs to the frequent category (often). The degree of infection in stick fish was 7.25 parasitic individuals per fish in the low category.
                        
                        
                        
                        
                            
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