Antibiotics are a class of prescription-only drugs used to treat bacterial infections. The use of antibiotics provides the expected therapeutic effects when prescribed and consumed rationally. However, irrational use of antibiotics can lead to antibiotic resistance, which is a serious global health issue. Antibiotic resistance reduces treatment effectiveness, prolongs disease duration, and increases the risk of mortality. This study aims to examine the relationship between the level of knowledge and compliance in antibiotic use among the congregation of Abdiel Zion Christian Church Denpasar in 2025. This study employs an observational design with a cross-sectional approach. A total of 74 respondents were selected using the consecutive sampling technique, and data were collected through questionnaires. Data analysis was conducted using the Chi-Square test with Jamovi software version 2.3.28.0. The results indicate that 70.3% of the congregation possesses a high level of knowledge about antibiotics, and 63.5% exhibit high compliance in antibiotic use. Data analysis reveals a significant relationship between knowledge level and compliance in antibiotic use, with a p-value <0.001 (p<0.05) and a contingency coefficient of 0.636. These findings suggest that the higher the level of knowledge about antibiotics, the higher the compliance in their use.
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