Indonesian Journal of Biotechnology
Vol 30, No 1 (2025)

Optimized bioethanol production from banana stem waste via simultaneous saccharification and fermentation with Saccharomyces cerevisiae

Neil Priharto (Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40135, Indonesia)
Agung Setiawan (Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40135, Indonesia
Deceased October 2023)

Dea Indriani Astuti (Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40135, Indonesia)



Article Info

Publish Date
29 Mar 2025

Abstract

Indonesia, one of the world’s largest banana producers, generates significant quantities of banana stem waste, leading to environmental challenges. This study explores the potential of converting this lignocellulosic biomass into bioethanol using a combination of steam pretreatment and simultaneous saccharification and fermentation (SSF) with Saccharomyces cerevisiae. The SSF process integrates enzymatic hydrolysis and fermentation, streamlining bioethanol production. The research applied the Taguchi method with an L9(34) orthogonal array to optimize key parameters, including enzyme concentration, particle size, temperature, and pH. Optimal conditions—5% enzyme concentration (v/v), 60 mesh banana powder, 35 °C and pH 5.00—yielded a maximum ethanol concentration of 9 g/L. Enzyme concentration and particle size were identified as critical factors in enhancing bioethanol yield. This study highlights the potential of banana stem waste as a sustainable resource for bioethanol production, contributing to waste reduction and renewable energy development.

Copyrights © 2025






Journal Info

Abbrev

ijbiotech

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...