Pengujian Efikasi Antimikroba Ekoenzim terhadap Staphylococcus aureus dan Escherichia coli sebagai Aktivitas Partisipatif Siswa dalam Materi Bioteknologi untuk Pengelolaan Limbah Makanan

Fina Nurul Arifah (Department of Science Education, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia)
Eka Cahya Prima (Department of Science Education, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia)

Symbiotic: Journal of Biological Education and Science
Vol. 6 No. 1 (2025): Symbiotic: Journal of Biological Education and Science Vol. 6 No. 1 April 2025

This study investigates the antimicrobial efficacy of eco-enzymes derived from organic waste, specifically fruit and vegetable peels, against Escherichia coli and Staphylococcus aureus bacteria. Eco-enzymes were produced through a simple fermentation process involving kitchen scraps, brown sugar, and water. Antibacterial activity was evaluated using agar diffusion assays, where different concentrations of eco-enzymes were tested against bacterial cultures. Results showed significant inhibition zones, indicating effective antimicrobial properties against both bacterial strains. Higher concentrations of eco-enzymes correlated with larger inhibition zones, highlighting their potential as natural alternatives for bacterial control in various applications, including food processing and wastewater treatment. Furthermore, eco-enzymes contribute to sustainable organic waste management by converting waste into useful bio-sanitizers, floor cleaners, bio-pesticides, and plant fertilizers. This research underscores the dual benefits of eco-enzymes in reducing environmental impact and promoting sustainable development goals.