International Journal of Multidisciplinary: Applied Business and Education Research
Vol. 6 No. 6 (2025): International Journal of Multidisciplinary: Applied Business and Education Rese

Box-Behnken Design-Based Optimization of Treatment Parameters for Soluble Reactive Phosphorus Removal of Synthetic Wastewater using Immobilized Spirulina platensis Beads

Calajate, Sean Andre D. (Unknown)
Robles, Francis Edric M. (Unknown)
Rojas, Maria Francesca I. (Unknown)
Tolentino, Tristan Josef A. (Unknown)
Masongsong, Angela Nicole S. (Unknown)
Estrellado, John Ray C. (Unknown)



Article Info

Publish Date
24 Jun 2025

Abstract

Soluble reactive phosphorus (SRP), a bioavailable phosphorus form, contributes to over-eutrophication by stimulating uncontrolled algal growth. This study aims to determine the optimum treatment parameters for the SRP removal from synthetic wastewater using the alginate-immobilized cyanobacteria Spirulina platensis. S. platensis was immobilized in alginate beads with varying alginate concentrations (2.5%, 3%, and 3.5% w/v), and subjected to varying operation time (1, 2, and 3 days), and bead dosage (1.5, 2, and 2.5 beads/mL) for SRP removal using Box-Behnken experimental design. Resulting model indicated a strong predictive relationship with R2 = 0.9253 and p = 0.0212. Main effects of bead dosage (p = 0.01372), its quadratic effect (p = 0.01643), and its interaction with alginate concentration (p = 0.00465) were found to be statistically significant. Predicted optimum parameters (2.5% w/v alginate, 3 days, and 1.5 beads/mL) were validated and resulted in a lower SRP removal of 92.80 ± 0.73% with a percent error of 5.22% relative to a predicted SRP removal of 97.91%. Extrapolation of the prediction model to 100% outside the experimental region was verified resulting in SRP removal of 97.39 ± 0.08% with a percent error of 2.61% was achieved by adjusting the operation time to 3.4 days. The study shows promising potential of immobilized S. platensis beads in addressing over-eutrophication through significant phosphorus reduction.

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Journal Info

Abbrev

ijmaber

Publisher

Subject

Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Environmental Science Medicine & Pharmacology

Description

International Journal of Multidisciplinary: Applied Business and Education Research is a peer-reviewed in a monthly basis that publishes full-length papers. it is to enhance the dissemination of knowledge across the multidisciplinary community. We are particularly interested in papers relevant to ...