Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram
Vol 13, No 3: July 2025

Isolation and Identification of Terpenoids from Artocarpus camansi Leaves Based on Column Chromatography and UV-IR Spectroscopy

Ramadani, Ramadani (Unknown)



Article Info

Publish Date
27 May 2025

Abstract

This study aims to isolate and identify terpenoid compounds from kluwih leaves (Artocarpus camansi). Extraction was done using methanol solvent, followed by n-hexane fractionation, which produced a concentrated blackish green extract. Compounds were separated through column chromatography with silica gel as the stationary phase and n-hexane as the mobile phase, and a mixture of n-hexane: ethyl acetate, producing eight fractions. The third fraction, obtained as green crystals, exhibited a positive reaction to the Liebermann-Burchard reagent, a specific test for the presence of terpenoid compounds. The observed colour change indicated the presence of terpenoids, making this fraction a promising candidate for isolation and detailed analysis. After recrystallisation, white needle-shaped crystals were obtained with a melting point of 141–142.8°C. Purity testing using thin layer chromatography (TLC) showed a single spot in various eluents, indicating that the isolated compound was pure. Further characterisation using UV spectroscopy showed maximum absorption at 210.2 nm, while IR spectroscopy showed the presence of OH, methyl, and non-conjugated C=C double bonds. Based on these data, the isolated compounds can be identified as terpenoid compounds. This study provides a basis for further studies on the structure and biological activity of kluwih leaves.

Copyrights © 2025






Journal Info

Abbrev

prismasains

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Mathematics Physics Other

Description

J-PS (Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram) was published by the Faculty of Science, Engineering, and Applied Science Universitas Pendidikan Mandalika. J-PS containing scientific articles in the form of research and ...