Jurnal Riset Sains dan Kimia Terapan
Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025

In Vitro Evaluation of the Antibacterial Effect of Virgin Coconut Oil Against Pseudomonas aeruginosa Associated with Folliculitis

Hati, Futi Kusuma (Unknown)
Rismaya, Rina (Unknown)
Aurellia, Dema Griseldis (Unknown)



Article Info

Publish Date
01 Jul 2025

Abstract

Virgin Coconut Oil (VCO) is a pure coconut oil rich in lauric acid, giving it potential antibacterial properties. Previous studies have successfully produced a VCO using a combined fermentation and enzymatic method with Neurospora sitophila, Lactobacillus plantarum, and papain. Nevertheless, further investigation is required to determine the antibacterial efficacy of the VCO against Pseudomonas aeruginosa, a bacterium responsible for folliculitis. This study analyzed of the antibacterial activity of VCO and Hydrolyzed Virgin Coconut Oil (HVCO) against P. aeruginosa in vitro. VCO is obtained by fermenting coconut milk using N. sitophila, L. plantarum and papain, then VCO undergoes hydrolysis with the aid of Lipozyme TL IM to yield HVCO. Inhibition zone assays were conducted to assess the antibacterial activity of 100% VCO and HVCO at varying concentrations of 25%, 50%, and 100%. The largest inhibition zone was shown by 100% HVCO (9.5 ± 0.3 mm in diameter), which was statistically significantly different from the control, ampicillin trihydrate. The data suggested that HVCO has enhanced antibacterial effectiveness against P. aeruginosa, compared to VCO. This increased activity is attributed to its higher free fatty acids and monolaurin content.

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Journal Info

Abbrev

jrskt

Publisher

Subject

Chemistry

Description

Jurnal Riset Sains dan Kimia Terapan (JRSKT) is the journal for the publication of original studies and occational reviews in the field of science and applied chemistry. It is published electronically two times a ...