Jurnal Biologi Tropis
Vol. 25 No. 3 (2025): Juli-September

Primer Construction of Transporter Genes Based on Almt1 for The Analysis of Cmp-Sialic Acid Transporter Gene in Derendan

Taufikurrahman, Taufikurrahman (Unknown)
Putri, Fatma Jumaita (Unknown)
Nurbaiti, Nurbaiti (Unknown)
Ramadhani, Putri Oktavia (Unknown)
Wahibah, Ninik Nihayatul (Unknown)



Article Info

Publish Date
09 Jul 2025

Abstract

Derendan (Lansium sp.) is an underutilized endemic fruit tree species from Bengkalis Island that grows in acidic peatland soils with high concentrations of toxic aluminum ions (Al³⁺). This study aimed to construct gene-specific primers to detect stress tolerance genes in derendan, with an initial focus on the ALMT1 (Aluminum-activated malate transporter) gene, which is known to mediate aluminum detoxification through organic acid exudation. Primers were designed based on ALMT1 sequences from model species and tested using PCR amplification and sequencing of derendan genomic DNA. Surprisingly, the amplified DNA fragment showed high similarity with CMP-sialic acid transporter 1 genes in several plant species, based on BLASTn analysis. The CMP-sialic acid transporter gene encodes a protein involved in the transport of sialic acid, an organic acid known to play roles in abiotic stress responses. These findings suggest that the constructed primers may target conserved transporter domains related to stress adaptation. The resulting primers have potential applications in molecular studies and breeding programs aimed at enhancing peatland stress tolerance in derendan and related species.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...