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All Journal AL KAUNIYAH Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Biologi Tropis PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat BIOVALENTIA: Biological Research Journal
Ninik Nihayatul Wahibah
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences

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POLIMORFISME PEROKSIDASE RAMIN (Gonystylus bancanus (Miq.) Kurz) DI HUTAN PT. DIAMOND RAYA TIMBER PROVINSI RIAU Mellyasari Pangkey; Ninik Nihayatul Wahibah; Nery Sofiyanti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Ramin is a peat swamp endemic plant highly demanded in furniture industries. The high price and market demand has led to increase the illegal logging which subsequently threaten ramin population existence. On the other hand, genetic related information on ramin is limited. Biochemical approaches such as isozyme can be used to analyze genetic diversity. The objective of this study was to reveal the peroxsidase (PER) polimorphism of ramin natural population at PT. Diamond Raya Timber (PT. DRT) forest in Riau Province. PER were analyzed using horizontal elektrophoresis model. The leaf samples that used in this study were collected from 50 individuals ramin. Data were analyzed using SAHN function and UPGMA method by NTSYS version 2.0. The results showed that PER enzyme produced clear visualization, forming 12 band patterns that were formed by six bands which was different migrating direction to the anode pole (positive) and catode pole (negative). The dendrogram in this study showed that 50 individuals of ramin were clustered into two main separated groups at the level of 47% similarity coefficient. These data indicated that the genetic variation of ramin in PT.DRT was quite diverse.
KEANEKARAGAMAN GENETIK RAMIN (Gonystylus bancanus Miq. Kurz) DI CAGAR BIOSFER GIAM SIAK KECIL-BUKIT BATU KABUPATEN BENGKALIS PROVINSI RIAU BERDASARKAN POLA PITA ISOZIM Meilian Syafitri; Ninik Nihayatul Wahibah; Siti Fatonah
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Ramin is timber plant with high demand in furniture industries because it has good woodand fiber texture. The high selling prices and market demand has led to increase illegallogging, consequently ramin population will be endangered. Therefore, it is necessary tostudy the genetic diversity as one of the conservation effort of ramin using biochemicalmarkers. This study was aimed to analyze the genetic diversity of ramin (Gonystylusbancanus Miq. Kurz) in Giam Siak Kecil-Bukit Batu Bengkalis biosphere reserves using anisozymes technique. Isozyme analysis was performed using the horizontal model of starchgel electrophoresis. A total of 50 individual ramins were used for analyzing the isozymebanding patterns using one enzyme namely peroxidase (PER). The result showed clearbanding patterns. PER enzyme produced twelve banding patterns and migrated to theanode (positive) and cathode (negative) poles. SAHN clustering analysis using UPGMAmethod showed that all of the individuals of ramin were clustered with the similaritycoefficient of 38% and separated into two groups (I and II). All of the members of firstcluster were grouped with the similarity coefficient 61%, while the second cluster memberswere grouped with similarity coefficient 55%. This data indicated that the genetic diversityof ramin in biosphere reserves Giam Siak Kecil-Bukit Batu Bengkalis Regency of RiauProvince was quite high.
Aplikasi Ecoprint untuk Mendukung Peningkatan Produktifitas Ekonomi Perempuan Desa Tarai Bangun: Ecoprint Application to Support Increasing Economic Productivity of Women in Tarai Bangun Village Wahibah, Ninik Nihayatul; Martina, Atria; Zul, Delita; Yulminarti, Yulminarti; Meiwanda, Geovani
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 4 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i4.7038

Abstract

Most women in Tarai Bangun Village focus on their domestic activities. The community service program aimed to empower women in the enhancing of their economic productivity through gaining new skills such as the eco print technique. Ecoprint is an eco-friendly product, using parts of plants; therefore, it also inspires women to preserve the existence of local germplasm. The results of the program showed that all participants were able to make ecoprint products. They also were satisfied with this program. However, the drawback of this program is that our partners need to understand marketing techniques.
Primer Construction of Transporter Genes Based on Almt1 for The Analysis of Cmp-Sialic Acid Transporter Gene in Derendan Taufikurrahman, Taufikurrahman; Putri, Fatma Jumaita; Nurbaiti, Nurbaiti; Ramadhani, Putri Oktavia; Wahibah, Ninik Nihayatul
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9069

Abstract

Derendan (Lansium sp.) is an underutilized endemic fruit tree species from Bengkalis Island that grows in acidic peatland soils with high concentrations of toxic aluminum ions (Al³⁺). This study aimed to construct gene-specific primers to detect stress tolerance genes in derendan, with an initial focus on the ALMT1 (Aluminum-activated malate transporter) gene, which is known to mediate aluminum detoxification through organic acid exudation. Primers were designed based on ALMT1 sequences from model species and tested using PCR amplification and sequencing of derendan genomic DNA. Surprisingly, the amplified DNA fragment showed high similarity with CMP-sialic acid transporter 1 genes in several plant species, based on BLASTn analysis. The CMP-sialic acid transporter gene encodes a protein involved in the transport of sialic acid, an organic acid known to play roles in abiotic stress responses. These findings suggest that the constructed primers may target conserved transporter domains related to stress adaptation. The resulting primers have potential applications in molecular studies and breeding programs aimed at enhancing peatland stress tolerance in derendan and related species.
Identification of Castor Genotypes Resistant to Graymold in Peatland Area based on Resistance Gene Analogs: A Preliminary Study Wahibah, Ninik Nihayatul; Atria , Martina; Arini, Arini; Yasir, Sidik
BIOVALENTIA: Biological Research Journal Vol. 10 No. 2 (2024)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.10.2.2024.236

Abstract

Graymold is one of devastating fungal disease of castor plants (Ricinus communis L.) caused by Botryotinia ricini. Resistant germplasm is the basic and essential sources to support castor breeding obtaining disease resistant varieties. Objective of this study was to identify castor genotypes resistant to gray mold in peatland agroforestry area in Kepulauan Meranti that naturally infected by graymold fungal pathogen.  This is the first report about castor plants cultivated under peat soil. Infected and uninfected castor plants were sampled and analyzed their Resistance Gene Analog (RGA).  Results showed that resistant plants expressed diverse phenotype and amplified DNA fragment using RGA primers. The fragments were similar to ABC transporter gene which is one of RGA classes. Additionally, the fragments revealed P-loop NTPase conserved domain. In contrast, all infected plants failed to produced PCR product.  The results indicated that uninfected castor plants can be used as source of resistant trait. Their RGA sequences can be analyzed to develop molecular marker for supporting gray mold resistant breeding program.
Endophytic Bacteria From Banana Plants Improves The Growth of Rice (Oryza sativa) and Maize Plants (Zea mays) Sidiq, Yasir; Silviani, Defina Anggita; Rahayu, Triastuti; Saputra, Alanindra; Wahibah, Ninik Nihayatul; Widianto, Donny
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.37884

Abstract

AbstractFive isolates of endophytic bacteria from the roots of klutuk and ambon banana plants produce indole acetic acid (IAA) hormone. However, the evidence of its potential as Plant Growth Promoting Bacteria (PGPB) has not been observed. Therefore, this study aims to determine the effect of bacterial isolate to increase the growth of rice and maize plants. The growth rates of bacterial isolates were measured before the inoculation into the plant root. The root inoculation was performed following a complete randomized design. Root inoculation was carried out using 5 isolates of endophytic bacteria in rice and maize plant seedlings, and the process was repeated 15 times (n=15). The growth of rice and maize plants was monitored from 1st day after planting (dap) to the 14th dap. The collected data were analyzed by student t-test with a significant level of 5% (a= 0.05). The results showed that the logarithmic phase of all isolates was reached at 4 to 16 hours and K10, K25, K35, K111, and A41 isolates increased the height of plants while the K111 isolate can significantly increase the plant height compared to the control. This experiment revealed that the endophytic bacteria from banana plants increase the rice and maize plant’s growth.AbstrakLima isolat bakteri menghasilkan hormon indole acetic acid (IAA) yang berpotensi memacu pertumbuhan tanaman. Namun, bukti empiris potensinya sebagai Plant Growth Promoter Bacteria (PGPB) belum didapatkan. Penelitian ini bertujuan untuk mengetahui pengaruh isolat bakteri terhadap peningkatan pertumbuhan padi dan jagung. Penelitian ini menggunakan metode eksperimen dengan desain rancangan acak lengkap dan setiap perlakuan diulang 15 kali (n= 15). Hasil pertumbuhan tanaman yang diinokulasi dengan isolat bakteri masing-masing dibandingkan dengan perlakuan kontrol. Sebelum perlakuan, tingkat pertumbuhan bakteri endofit diukur sehingga didapatkan fase logaritmik yang cocok untuk dilakukan inokulasi ke akar tanaman. Setelah perlakuan, pertumbuhan tanaman padi dan jagung dipantau dari hari ke-1 setelah tanam (hat) sampai dengan ke-14 hat. Data dianalisis dengan uji t dengan taraf signifikan 5% (α= 0,05). Hasil penelitian menunjukkan bahwa 5 isolat yang berasal dari tanaman pisang mengalami fase logaritmik pada masa inkubasi 4 hingga 16 jam dan semua isolat bakteri meningkatkan tinggi tanaman serta isolat K111 dapat meningkatkan tinggi tanaman secara signifikan dibandingkan dengan kontrol. Penelitian ini mengungkapkan kemampuan bakteri endofit dari tanaman pisang untuk meningkatkan pertumbuhan tanaman.
Co-Authors Alanindra Saputra Arini Arini Atria , Martina Atria Martina Delita Zul Donny Widianto Geovani Meiwanda Meilian Syafitri Mellyasari Pangkey Nery Sofiyanti Nurbaiti Nurbaiti Purwoko, Agus Putri, Fatma Jumaita Ramadhani, Putri Oktavia Silviani, Defina Anggita Taufikurrahman Taufikurrahman Triastuti Rahayu Yasir Sidiq Yasir, Sidik Yulminarti, Yulminarti