Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering)
Vol. 14 No. 4 (2025): August 2025

Potential of Foliar Fertilizer and Organic Additives for Vanda tricolor Protocorm Regeneration

yulaekhah, siti (Unknown)
Herawati, Maria Marina (Unknown)
Pudjihartati, Endang (Unknown)



Article Info

Publish Date
25 Jul 2025

Abstract

Vanda tricolor orchid is propagated by in vitro culture using Vacin & Went (VW) media. The price of this media is quite high, so research is needed on alternative media that can replace VW media. The objectives of this study were to (1) determine the effect of foliar fertilizer concentration and addition of organic materials on in vitro protocorm regeneration in to planlets, (2) obtain a combination of foliar fertilizer concentration and organic materials that can be used to subtitude VW as a medium for Vanda tricolor protocorm regeneration. The treatments tested were the concentration of NPK foliar fertilizer (21:21:21), namely:     1 g/L, 1.5 g/L, 2 g/L, and 2.5 g/L and various organic materials, namely: no organic material, banana, potato, banana+potato. VW was use as a control. Data analysis to compare VW control with all treatments used Orthogonal Contrast further test, while to compare between treatment combinations using Analysis of Variance (ANOVA) and DMRT further test at the 5% level. The results showed that leaf fertilizer at a concentration of 2.5 g/L was able to accelerate the formation of shoots and roots without the addition of organic material. In addition, leaf fertilizer media plus potatoes can be used to replace VW as a protocorm regeneration media at affordable cost.

Copyrights © 2025






Journal Info

Abbrev

JTP

Publisher

Subject

Agriculture, Biological Sciences & Forestry Engineering

Description

Jurnal Teknik Pertanian Lampung or Journal of Agricultural Engineering (JTEP-L) is a peer-reviewed open-access journal. The journal invites scientists and engineers throughout the world to exchange and disseminate theoretical and practice-oriented researches in the whole aspect of Agricultural ...