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All Journal HAYATI Journal of Biosciences Agrotrop : Journal on Agriculture Science AGRIKA Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Karet Jurnal Ilmiah Pertanian Caraka Tani: Journal of Sustainable Agriculture Jurnal SOLMA JMM (Jurnal Masyarakat Mandiri) Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Abdi Insani Proceedings Series on Physical & Formal Sciences Warta LPM AGRIC

Endang Pudjihartati

Universitas Kristen Satya Wacana

Author-ID : 3030717

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE Herawati, Maria Marina; Pudjihartati, Endang; Pramono, Suwijiyo; Sulistyaningsih, Endang; Purwantoro, Aziz
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.Keywords: 2,4-D, Artemisia cina, BA, polyploidy

ISOLASI, IDENTIFIKASI DAN ANTAGONISME IN VITRO ISOLAT TRICHODERMA SPP. ASAL KEBUN KARET BLIMBING, PEKALONGAN, JAWA TENGAH Berlian, Intan; Anarqi, Sindu; Pudjihartati, Endang
Jurnal Penelitian Karet JPK : Volume 34, Nomor 2, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i2.231

Rigidoporus microporus merupakan patogen penyebab penyakit jamur akar putih (JAP) yang sangat merugikan perkebunan karet di Kebun Blimbing, Pekalongan, Jawa Tengah. Tindakan pengendalian secara preventif, kuratif dengan cara kultur teknis dan kimiawi sudah dilakukan namun belum dapat mengendalikannya. Penelitian ini bertujuan untuk mendapatkan isolat lokal perkebunan karet di Kebun Blimbing yang bersifat antagonis terhadap JAP. Penelitian terdiri dari: (1) isolasi, (2) identifikasi, dan (3) uji antagonisme dengan metode dual culture. Pengujian menggunakan rancangan acak lengkap. Penelitian ini berhasil mendapatkan sebelas isolat Trichoderma spp. dari rhizosfer tanaman karet di Kebun Blimbing. Sebelas isolat tersebut yaitu Trichoderma spp. dari Blok Sikaum TBM 1 (P1), Blok Sikaum TBM 2 (P2), Blok Sikaum TM 1 (P3), Blok Sikaum TM 2 (P4), Blok Sari TM 1 (P5), Blok Sari TM 2 (P6), Blok Sitirejo TBM 1 (P7), Blok Sitirejo TBM 2 (P8), Blok Semurup TM 1 (P9), Blok Semurup TM 2 (P10), Blok Semurup TM 3 (P11). Semua isolat Trichoderma spp. tersebut bersifat antagonis terhadap pertumbuhan R. microporus. Persentase daya hambat Trichoderma spp. terhadap R. microporus berkisar antara 36,56% sampai 69,66%. Penghambatan paling tinggi (69,66%) diamati pada isolat P9 dan terendah (36,56%) pada isolat P3.

Bio-Priming Benih Kedelai (Glycine Max (L.) Merrill) untuk Meningkatkan Mutu Perkecambahan Livia Trihanni Hasan, Theresa Dwi Kurnia Endang Pudjihartati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 2 (2016): June 2016
Publisher : Universitas Atma Jaya Yogyakarta

Sifat benih kedelai dengan kandungan protein dan lemak tinggi menjadi penyebab benih kedelai cepat mengalami deteriorasi atau penurunan mutu benih. Salah satu cara untuk meningkatkan mutu perkecambahan adalah dengan perlakuan pemeraman atau bio-priming. Tujuan penelitian ini adalah mengetahui pengaruh bio-priming dalam meningkatkan mutu perkecambahan. Penelitian ini menggunakan benih kedelai kuning varietas Grobogan yang sudah disimpan selama tiga bulan. Rancangan percobaan menggunakan Rancangan Acak Kelompok (RAK) dan data yang diperoleh dianalisis sidik ragam dengan uji Beda Nyata Jujur (BNJ) taraf signifikansi 5%. Perlakuan dalam penelitian ini adalah: 1) Kontrol (K), 2) Priming (M) perlakuan matriks priming dengan memasukkan benih kedelai ke dalam media berupa arang sekam lembab dengan perbandingan benih : arang sekam : air = 3 : 10 : 3 (b/b/v), 3) Bio-priming menggunakan EM-4 (E) dengan cara merendam benih dalam larutan EM-4 0.3% selama satu jam, 4) Bio-priming menggunakan Trichoderma harzianum (T) yaitu priming benih dengan 75 g Trichoderma harzianum selama satu hari, dan 5) Bio-priming menggunakan EM-4 + Trichoderma harzianum (ET). Hasil penelitian menunjukkan bahwa bio-priming dengan menggunakan EM-4 mampu memperbaiki viabilitas, vigor dan pertumbuhan kecambah kedelai, sedangkan bio-priming menggunakan Trichoderma harzianum tidak menunjukkan perbedaan nyata pada semua variabel pengamatan dibandingkan kontrol. Perlakuan bio-priming menggunakan kombinasi EM-4 dan Trichoderma harzianum cenderung menurunkan mutu perkecambahan kedelai dibandingkan kontrol.

Embriogenesis Somatik dari Eksplan Benih Gandum Tropis (Triticum aestivum L.) Pudjihartati , Endang; Marina Herawati , Maria
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Embriogenesis somatik dalam penelitian ini menggunakan eksplan embrio dewasa (benih) gandum tropis varietas Dewata dan galur R-036 dan R-040 koleksi Pusat Studi Gandum Fakultas Pertanian dan Bisnis UKSW, meliputi tahap induksi dan proliferasi. Penelitian ini bertujuan mendapatkan genotipe gandum tropis yang mudah di kultur secara in vitro dan medium yang baik dalam rangka memperoleh Embrio Somatik (ES). Pada tahap induksi, benih gandum dikultur pada empat konsentrasi 2,4-d (2; 2,5; 3 dan 3,5 ppm). Pada tahap proliferasi diteliti empat komposisi media yang mengandung 2,5 dan 3 ppm 2,4-d dengan dan tanpa 0,5 ppm BAP. Hasil penelitian menunjukkan bahwa jumlah ES dan skor ES primer relatif tertinggi dari varietas Dewata pada 3 ppm 2,4-d, galur R-040 pada 2,5 ppm 2,4-d, dan galur R-036 pada 2 ppm 2,4-d. Eksplan gandum galur R-036 menghasilkan jumlah ES dan skor ES primer relatif tertinggi dibandingkan genotipe lain. Mutu benih yang digunakan mempengaruhi respons eksplan dalam menghasilkan jumlah ES dan skor ES primer dan kurang mempengaruhi jumlah ES dan skor ES sekunder. Pada tahap proliferasi, jumlah ES sekunder relatif tertinggi, dari galur R-036 pada 2,5 ppm 2,4-d, sedangkan varietas Dewata dan R-040 berturut-turut pada 2,5 ppm dan 3,0 ppm 2,4-d. Aplikasi 2,4-d yang dikombinasikan dengan BAP dapat menurunkan jumlah ES, tetapi hanya cenderung menurunkan skor ES sekunder pada ketiga genotipe. Pada skor ES hasil proliferasi tidak diamati adanya pengaruh komposisi ZPT yang diteliti (2,4-d dan BAP) pada varietas Dewata dan galur R-040, sedangkan pada galur R-036 skor ES sekunder maksimum pada 2,5 ppm 2,4-d.

Chitinase Activity in Healthy and Sclerotium rolfsii Infected Peanut ENDANG PUDJIHARTATI; SISWANTO SISWANTO; SATRIYAS ILYAS; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

The objectives of this experiment were to analyze the endo- or exo-chitinase activities of healthy and Sclerotium rolfsii infected peanuts. The experiment analyzed 24 different peanut genotypes. Results of the experiment showed chromogenic dimer was the most suitable substrate for analysing chitinase activities. Both endo- and exo-chitinases activities were detected in leaf, stem, and crown tissues. Increased in chitinase activities were detected in S. rolfsii infected peanut tissues than in healthy plant. Regression analysis showed negative slope between disease intensity and chitinase activity in S. rolfsii infected peanut tissue (R2= 0.45). Key words: Disease response, stem rot disease, endo- and exo-chitinase

Oxidative Burst, Peroxidase Activity, and Lignin Content of Sclerotium rolfsii Infected Peanut Tissue ENDANG PUDJIHARTATI; SATRIYAS ILYAS; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

The objectives of this experiment were to analyse physiological responses, such as oxidative burst reaction, peroxidase activity, and lignin content of healthy and S. rolfsii-infected peanut tissues. Differences in physiological responses among 24 peanut genotypes were determined, the disease severity was calculated and used to group resistance of tested genotypes. The regressions among observed peroxidase activity, lignin content and disease severity were used to determine the possible mechanisms of S. rolfsii resistance in peanut. Peanut seeds were grown in polybag and the growing plants were inoculated at the crown, stem, and leaf tissues. Results of the experiment indicated that infection of S. rolfsii in peanut did not induce oxidative burst. However, infection of the pathogen resulted in increased peroxidase activity and lignin content in the infected tissues. Regression analysis between peroxidase activity and disease severity showed negative slopes, indicating the more resistance the genotype, the more peroxidase activity in the tissue. Regression analysis between lignin content and disease severity was not significant. Key words: hypersensitive response (HR), resistance mechanisms, Sclerotium stem rot, disease response, Arachis hypogaea

Pengaruh Pemangkasan Cabang dan Mikoriza terhadap Produksi dan Mutu Benih Mentimun (Cucumis Sativus L.) ANWI APRILIANA; ENDANG PUDJIHARTATI; HARMASTINI SUKIMAN
Agrotrop : Journal on Agriculture Science Vol 9 No 1 (2019)
Publisher : Fakultas Pertanian Universitas Udayana

The Effect of Branch Pruning and Mycorrhiza on Production and Seed Quality of Cucumis sativus L. The purpose of this study was to obtain the best branch pruning, with or without mycorrhiza application, in order to increase the production and quality of cucumber seeds. Pruning the branch at the beginning of cucumber growth is expected to delay fruit formation and support good vegetative growth. This research was carried out in the PT. Primasid Andalan Utama seed production area in Kalibeji Village, Tuntang District, Semarang Regency, Seed Technology Laboratory and Plant Physiology Laboratory, Faculty of Agriculture and Business, Satya Wacana Christian University. This study was conducted by the split-plot randomized complete block design with 2 factors. The main plot consists of 2 levels, namely control/without mycorrhiza (M0) and mycorrhiza application (M1). Pruning the branches as the subplots consist of 4 levels, were pruning to the second, third, fourth and fifth branches of the cucumber plant (P1, P2, P3 and P4). DMRT was used to differentiate the significance of the treatment. The results showed that mycorrhiza application increased the percentage of root infections. The pruning to the fifth branch (P4) is the best; in control without mycorrhiza (M0P4) increased for the number of fruits, the seed weight per plant, and the germination simultaneously; whereas in mycorrhiza application (M1P4) increased the number of fruits and the seed weight per plant. It is also observed that the pruning to the second branch (P1) increased the weight of 1000 grains, the speed of germination and the simultaneous of germination; both with and without mycorrhiza application.

Pengaruh Perlakuan Organomatrixpriming Terhadap Peningkatan Mutu Fisiologis Benih Cabai (Capsicum annuum L.) Fatma Rosita Ratnaningtyas; Endang Pudjihartati
Buletin Anatomi dan Fisiologi Volume 4, Nomor 1, Tahun 2019
Publisher : Departemen Biologi Universitas Diponegoro

Penelitian ini bertujuan untuk mengetahui pengaruh organomatrixpriming dalam memperbaiki mutu fisiologis benih cabai. Benih yang digunakan adalah benih cabai varietas Ciko yang telah disimpan selama dua tahun dengan daya berkecambah 74,67%. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan faktor tunggal. Terdapat delapan perlakuan dan lima ulangan. Bahan organomatrixpriming yang diujikan adalah akuades, air kelapa, pupuk organik cair plus, tauge, tauge fermentasi, bonggol pisang, bonggol pisang fermentasi. Organomatrixpriming dilakukan menggunakan media zeolit selama 4 hari, kemudian dilanjutkan dengan pengujian perkecambahan menggunakan metode UKDdp. Hasil penelitian menunjukkan bahwa pemberian perlakuan pupuk organik cair plus dan bonggol pisang fermentasi dapat memperbaiki viabilitas benih cabai serta memenuhi standar daya berkecambah benih menurut Balitsa dan SNI yaitu ≥85%. Tetapi organomatrixpriming yang lain tidak berpengaruh nyata terhadap peningkatan viabilitas dan vigor benih. Perlakuan tauge fermentasi justru menurunkan seluruh variabel mutu fisiologis. Infeksi Aspergillus yang menyebabkan benih busuk dan tumbuh abnormal saat pengujian perkecambahan paling tinggi terdapat pada perlakuan tauge. Kata kunci : benih cabai, organomatrixpriming, viabilitas, vigor

The Effect of Phosphorus and Potassium on the Growth and Quality of Viola (Viola cornuta L.) Seed Production Alfa Sada Saputra; Suprihati Suprihati; Endang Pudjihartati
Caraka Tani: Journal of Sustainable Agriculture Vol 35, No 1 (2020): April
Publisher : Universitas Sebelas Maret

Viola cornuta L. is an ornamental plant that is primarily propagated by high-quality seeds that have high demand in subtropical countries. The use of fertilizers according to recommendations results in the growth of parent plants below the standard. Furthermore, seed production is generated below 2 g per plant and the seed germination capacity is below 80%. In addition, phosphorus (P) and potassium (K) nutrients can increase the growth and seed production of the plants, including ornamental plants. The study aimed to determine the influence of P and K nutrients on growth and seed production of V. cornuta L. Research was carried out in November 2018 - January 2019 at PT. Selektani Horticulture, Ngablak, Magelang. There were seven treatments with four replications. Experiments were arranged according to the Randomized Complete Block Design (RCBD). Plants were grown in 5l volume-polybags. Fertilization was done by watering the planting media. Analysis of variance was performed at 95% accuracy. The study shows that P and K nutrients have a highly significant effect on height, number of flowers, number of seed per capsule, seed production and germination energy and significantly affected the number of leaves. The addition of both P and K fertilizers in the vegetative and P in the generative stage (P6) can result in the productivity of 3.28 g plant-1 and produce germination energy ≥90%. It is recommended for increasing growth and seed production.

Optimalisasi metode uji perkecambahan dan media tanam pada perkecambahan biji anuma (Artemisia annua L.) Murtiwulandari; Endang Pudjihartati
Jurnal Ilmiah Pertanian Vol. 19 No. 3 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i3.10514

Annual wormwood (Artemisia annua L.) is a traditional Chinese medicinal plant belonging to a group of tiny seeds. The small size of the seeds requires the appropriate germination test method and medium. This study aimed to find the best seed germination test methods and growing media; also the best combination of methods and germination media for determining the viability and vigor of A. annua seeds. The experiment used a factorial randomized block design repeated three times, given two treatments: the type of method and the media. The methods used were the top-of-the-paper test (UDK), the between-paper test (UAK), and the rolled paper and erected-in-plastic test (UKDDdp). The media used included garden soil (control), straw paper, filter paper, CD paper, tissue towel, and soft tissue, so there were 15 treatment combinations and one control. Data analysis used a SAS and DMRT follow-up test with a 5% significance level. The results showed that of the observation variables for germination percentage (DB) and growth speed (KCT), the UDK test method became the best method. As a result of the main effect of media, filter paper became the best medium, while straw paper became the lowest after soil. In the seed viability testing, there was no interaction for a combination of methods and media, but only the UDK combination of filter paper reached DB=80%. Meanwhile, there was an interaction in the vigor test of the simultaneous growth (KST), and the best combination was to use the UDK method with towel tissue and soft tissue.

Co-Authors Alfa Sada Saputra Alfred Jansen Sutrisno Aminuyati Andree Wijaya Setiawan ANWI APRILIANA Aziz Purwantoro Bistok Hasiholan Simanjuntak Cornelia Deserinda Devita Kotten Djoko Murdono Endang Sulistyaningsih Erik Kado Nugroho Erlitha Rahmawati Exvaricha Exvaricha Fatma Rosita Ratnaningtyas Flora Novi Shania Fransisca Mareta Kurnia Sari HARMASTINI SUKIMAN Intan Berlian, Intan Jonathan Galih Raka Kusuma Kezia Natalia Lois Hintanara Shine Tanggara Maria Marina Herawati Murtiwulandari Novinanto, Antonius Nugraheni Widyawati Purwoko, Agus Rahotni Imanta Damanik Sabrina Ayu Satriyas Ilyas Sindu Anarqi, Sindu Sudarsono Suprihati Suprihati Suprihati Suwijiyo Pramono Teguh Iman Santoso Theresa Dwi Kurnia Tinjung Mary Prihtanti Willy Arnold Daniel Sekewael Yeni Setiyawan Yohanes Hendro Agus yulaekhah, siti Yuli Kristiyanto Yuliana Pratiwi Asti Yuliawati

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