G-Tech : Jurnal Teknologi Terapan
Vol 9 No 3 (2025): G-Tech, Vol. 9 No. 3 July 2025

Potential and Characterization of Yeast from Soil Contaminated with Captan – Based Fungicide

Putri, Ratna Eka Sari (Unknown)
Fatiha, Chosa Zahro (Unknown)
Putri, Novia Dwi (Unknown)



Article Info

Publish Date
10 Jul 2025

Abstract

Captan is a fungicide that inhibits the growth of fungi such as Alternaria porri. This fungicide is non-systemic, forming a layer on the surface of leaves and fruit. The continuous use of captan fungicides can leave residues in the environment, especially in soil. One technique to reduce captan fungicide residues is bioremediation. Bioremediation is a way to remove fungicide residues by utilizing microorganisms. The purpose of the study was to obtain yeasts in land polluted with residues of fungicides made from active captan, knowing yeasts that are adaptive to various fungicide concentrations and have the potential to reduce the toxicity of fungicides made from active captan. This study includes soil sampling, isolation and characterization of yeasts morphologically and molecularly, yeast adaptation tests to fungicides, and degradation tests using a complete randomized design. In the adaptation test, all yeasts were able to grow on media added with various concentrations of fungicide product recommendations. Molecular identification of yeast includes DNA isolation, DNA amplification, PCR product sequencing, and molecular data analysis. In the treatment with the addition of yeast, the largest average diameter of A. porri on the seventh day of observation was in the treatment with the addition of W. anomalus and W. onychis yeast. The yeast with the highest ability to degrade fungicides containing the active ingredient captan is W. onychis.

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Journal Info

Abbrev

g-tech

Publisher

Subject

Computer Science & IT Decision Sciences, Operations Research & Management Energy Engineering

Description

Jurnal G-Tech bertujuan untuk mempublikasikan hasil penelitian asli dan review hasil penelitian tentang teknologi dan terapan pada ruang lingkup keteknikan meliputi teknik mesin, teknik elektro, teknik informatika, sistem informasi, agroteknologi, ...