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Endophytic Fungi as Potential Agents of Helminthosporium sp.  in Rice Plant (Oryza sativa L.) Ratna Eka Sari Putri; Syamsudin Djauhari; Mintarto Martosudiro
Journal of Tropical Plant Protection Vol 2, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.2.3

Abstract

Brown spot disease is the most serious rice disease due to its destructive nature, wide distribution and presence of several physiological races of the organism. In Asia, the disease has been recorded to reduce crop yields vary broadly from 6-90%. The application of endophytic fungi is reported to increase plant resistance from the secondary metabolites produced. The resulting metabolites are synthesized compounds to maintain their existence in interacting with the environment. The aim of this study was to obtain endophytic fungi of rice plants that were able to suppres Helminthosporium sp. pathogens. Isolation of endophytic fungi was carried out from the roots, peniles, leaves and leaf sheath of rice plants. Identification of endophytic fungi was based on their macroscopic, microscopic characters and molecular identification. Endophytic fungi that had the highest inhibition were T. asperellum, F. nirenbergiae, and F. oxysporum as indicated by the in vitro antagonism test. T. asperellum, F. nirenbergiae, and F. oxysporum also produced IAA hormone between 1.8 to 4.365 mg/L.
Membentuk Generasi Hijau: Implementasi P5 Tema Gaya Hidup Berkelanjutan melalui Pembuatan Ecoenzym dan Pengolahan Sampah Organik oleh Pelajar SMA Negeri 1 Sukatani Sisi Handini, Aline; Madusari, Sylvia; Adistya Putri, Halida; Prilaningrum Sutanto, Oentari; Farida, Naimatul; Sari Putri, Ratna Eka; Gemilang Simanjuntak, Rich
ABDINE: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2025): ABDINE : Jurnal Pengabdian Masyarakat
Publisher : Sekolah Tinggi Teknologi Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52072/abdine.v5i1.1301

Abstract

Proyek Penguatan Profil Pelajar Pancasila (P5) diatur dalam Permendikbudristek Nomor 56 Tahun 2022 dalam implementasi Kurikulum Merdeka. Kegiatan P5 bertujuan dalam membentuk peserta didik khususnya pada SMA Negeri 1 Sukatani penanaman karakter dari nilai-nilai yang terkandung dalam profil pelajar Pancasila. Salah satu karakter dalam profil pelajar Pancasila yaitu Gaya Hidup Berkelanjutan dengan implemntasi menjaga lingkungan sekitar. Hal ini menjadi fokus untuk pengabdian masyarakat yang akan diadakan yaitu Pembuatan Ecoenzyme dan pengolahan sampah organik yang berkesinambungan dengan profil pelajar Pancasila tersebut. Pengolahan limbah organik ini memiliki banyak keunggulan dan memberikan pemahaman tentang zero waste bagi siswa SMA Negeri 1 Sukatani. Metode pelaksanaan pengabdian adalah pemaparan materi, persiapan maupun pengolahan ecoenzyme dan sampah organik secara langsung. Hasil yang didapatkan dalam pengabdian masyarakat ini adalah penyuluhan tentang pengolahan limbah organic yang ada dilingkungan sekolah sehingga dapat dimanfaatkan dalam kehidupan sehari-hari yang mendukung Gaya Hidup Sehat dari profil pelajar Pancasila
Potential and Characterization of Yeast from Soil Contaminated with Captan – Based Fungicide Ratna Eka Sari Putri; Chosa Zahro Fatiha; Novia Dwi Putri
G-Tech: Jurnal Teknologi Terapan Vol 9 No 3 (2025): G-Tech, Vol. 9 No. 3 July 2025
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v9i3.7253

Abstract

Captan is a fungicide that inhibits the growth of fungi such as Alternaria porri. This fungicide is non-systemic, forming a layer on the surface of leaves and fruit. The continuous use of captan fungicides can leave residues in the environment, especially in soil. One technique to reduce captan fungicide residues is bioremediation. Bioremediation is a way to remove fungicide residues by utilizing microorganisms. The purpose of the study was to obtain yeasts in land polluted with residues of fungicides made from active captan, knowing yeasts that are adaptive to various fungicide concentrations and have the potential to reduce the toxicity of fungicides made from active captan. This study includes soil sampling, isolation and characterization of yeasts morphologically and molecularly, yeast adaptation tests to fungicides, and degradation tests using a complete randomized design. In the adaptation test, all yeasts were able to grow on media added with various concentrations of fungicide product recommendations. Molecular identification of yeast includes DNA isolation, DNA amplification, PCR product sequencing, and molecular data analysis. In the treatment with the addition of yeast, the largest average diameter of A. porri on the seventh day of observation was in the treatment with the addition of W. anomalus and W. onychis yeast. The yeast with the highest ability to degrade fungicides containing the active ingredient captan is W. onychis.
Increase in Growth N, P, and K Absorption and Production of Corn (Zea mays L.) on Rainfed Paddy Fields Through Application of NPK 16-16-16 Fertilizer Chosa Zahro Fatiha; Retno Suntari; Ratna Eka Sari Putri
G-Tech: Jurnal Teknologi Terapan Vol 9 No 3 (2025): G-Tech, Vol. 9 No. 3 July 2025
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v9i3.7270

Abstract

One of the national strategic foodstuffs is maize, whose production can still be increased because the actual yield in the lower than the potential yield. Problems that often arise is low fertility, especially nutrients N, P, and K. The aims of this research is to know the effect of NPK 16:16:16 fertilizer application on the growth, production and uptake of corn plants. The research was conducted in Mojokerto rainfed paddy fields with a randomized group design. The treatments given are 8 in the form of different doses of NPK 16:16:16 fertilizer and control in the form of no fertilizer and NPK 15:15:15 according to the recommended dose. Observation parameters observed were soil analysis, number of leaves, plant height, plant dry weight, NPK nutrient uptake and weight of 100 grains. Data analysis used ANOVA f test at 5% level and DMRT further test at 5% level. The application of NPK 16-16-16 fertilizer at 1/4 dose significantly increased the growth of corn plant and nutrient uptake. NPK 16:16:16 fertilizer at 1/2 dose significantly increased production.