Keluwih: Jurnal Sains dan Teknologi
Vol. 6 No. 1 (2025): Keluwih: Jurnal Sains dan Teknologi (February) - In Progress

Study on the Banana Peel Hydrolysate for Lactic Acid Bacteria Growth Media

Akbarningrum Fatmawati (Unknown)
Endang Srihari (Unknown)
Puguh Setyopratomo (Unknown)
Rudy Agustriyanto (Unknown)



Article Info

Publish Date
28 Jul 2025

Abstract

Abstract—Banana peel is food waste material that may contain certain beneficial nutrition and be useful for bacterial media production. This experiment aims to determine the effect of several variables which may have influence on the ability of banana peel to support probiotic growth. The thermal treatment used autoclave. In the hydrolysis step for probiotic growth, the variables studied were pH and temperature. The best result of hydrolysis was obtained by the initial steam treatment, followed by hydrolysis process at pH 4.5 and 95oC. The reducing sugar content obtained was 0.96 g/L. In the fermentation process, pure banana peel hydrolysate media was able produces to support Lactobacillus plantarum growth better than mixed media. The pure banana peel hydrolysate media could achieve up to 12.89 log CFU/ml. The amylase activity produced in the pure banana peel hydrolysate media was 8.14 DP. Keywords: banana, probiotic, hydrolysis, growth, amylase Abstrak—Kulit pisang merupakan bahan limbah yang memiliki kandungan nutrisi menguntungkan dan berguna untuk produksi media pertumbuhan bakteri. Penelitian ini bertujuan menentukan pengaruh beberapa variabel yang dapat mempengaruhi kulit pisang untuk mendukung pertumbuhan probiotik. Kulit pisang tersebut diolah melalui hidrolisis enzimatik menggunakan enzim amylase termofilik. Variabel sebelum proses hidrolisis adalah penggunaan pengolahan termal menggunakan uap air dan tanpa pengolahan. Pada langkah hidrolisis, variabel yang dipelajari adalah pH dan suhu. Hasil hidrolisa terbaik adalah dengan penggunaan pengolahan awal dengan uap air diikuti dengan hidrolisis pada pH 4,5 dan suhu 95oC. Kandungan gula pereduksi yang diperoleh sebesar 0,96 g/L. Pada proses fermentasi, media dari hidrolisat kulit pisang murni dapat menghasilkan pertumbuhan Lactobacillus plantarum sampai 12,89 log CFU/mL. Aktivitas amylase yang dihasilkan dari media hidrolisat kulit pisang tersebut adalah 8,14 DP. Keywords: pisang, probiotic, hydrolysis, growth, amylase

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Journal Info

Abbrev

saintek

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering

Description

Keluwih: Jurnal Sains dan Teknologi (Keluwih: Journal of Science and Technology) is an online, open access, and peer-reviewed journal. JST publishes its issues twice in a year (February and Agustus). This journal is to provide a forum for the sharing, dissemination, and discussion of original ...