<![CDATA[The increasing demand for fossil fuel energy (BBM) has driven the development of renewable energy sources, one of which is biodiesel derived from microalgae. Chlorella sp. is a promising microalga due to its high lipid content. This study aims to determine the optimal concentration of tempeh wastewater for culturing Chlorella sp. to maximize lipid production as a raw material for biodiesel. The culture was carried out for five days using different treatments of tempeh wastewater: 0% (control using Walne fertilizer), 3%, 5%, 7%, and 9%. Observed parameters included temperature, pH, dissolved oxygen, nitrate, phosphate, cell density, and lipid content, with lipid extraction performed using the Soxhlet method. The culture temperature ranged from 25-27°C, pH increased from acidic to neutral, and dissolved oxygen levels decreased at higher wastewater concentrations. The highest cell density was found in the 7% treatment (8.45 million cells/mL), although it was not significantly different from the other treatments. The highest lipid content was obtained at 3% wastewater (40.3%), which was higher than the control (21.3%) and other treatments. The decrease in lipid content at higher wastewater concentrations was attributed to toxicity and reduced media quality. It is concluded that a 3% concentration of tempeh wastewater is optimal for enhancing lipid production in Chlorella sp. as a sustainable biodiesel feedstock.]]>
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