<![CDATA[Tubifex worms are freshwater oligochaetes characterized by their bright red color and high nutritional content, consisting of 57% protein, 13.3% fat, 2.04% crude fiber, and 3.6% ash. Their natural populations have declined due to water pollution from waste, which reduces water quality, thus necessitating cultivation efforts. This study aimed to evaluate the effect of fermented sawdust and cocopeat as culture substrates on the biomass, population, and growth rate of Tubifex worms. The experiment was conducted using a Completely Randomized Design (CRD) with a non-factorial model consisting of four treatments and three replications: A (control: 80% mud + 20% chicken manure), B (80% fermented sawdust + 20% chicken manure), C (80% fermented cocopeat + 20% chicken manure), and D (40% fermented sawdust + 40% fermented cocopeat + 20% chicken manure). Analysis of variance (ANOVA) showed that different substrate compositions had a significant effect on biomass, population peak, and growth rate. The best performance was observed in treatment B, which resulted in the highest biomass (13.32 g/container), the highest population peak (120,111 individuals/container on day 40), and the highest growth rate (3.09%). These results indicate that fermented sawdust supplemented with chicken manure is the most effective substrate for enhancing the growth and population of Tubifex worms.]]>
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