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Hormonal induction on artificial ovulation and spawning of striped catfish, Pangasianodon hypopthalmus (Sauvage, 1878) using aromatase inhibitor and oxytocin Mahdaliana Mahdaliana; Agus Oman Sudrajat; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 16 No 1 (2016): February 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i1.46

Abstract

Artificial spawning on stripe catfish has generally carried out by stripping because of the absence in reflex of spawning. Mechanical stripping usually caused stress, decreased quality of gametes and seeds and damage on gonad. Induced spawning without stripping could be used for the process of gonad maturation to stimulate the ovulation of the fish which has the difficulties to spawn in its non natural habitat. Induced spawning without stripping by using hormones combi-nation was conducted in the present study. This research proposed to evaluate the injection of hormones combination between aromatase inhibitor (AI) and oxytocin association with ovaprim and PGF2a for stimulating ovulation and spawning without stripping. The completely randomized design of combined hormones consisted of five treatments such as P1(AI,oxytocin), P2 (AI, oxytocin,ovaprim), P3 (AI, oxytocin, ovaprim, PGF2a), P4 (ovaprim) as positive control, and P5 (NaCl) as negative control. Each treatment was performed using five paires of males and females as individual replicate of about 2-6 kg weight. The results showed that the combination of P3 was the most effectively and successfully induced ovulation with naturally spawning without stripping, treatment P4 ovulation with stripping, while the treatment P5 there was not ovulation. The hormones combination caused decreasing of estradiol-17p concentration and testosterone (p<0.05) as the sign of the final maturation. The average time of ovulation was 12.35±4.05 to 15.20±2.25 minutes. The highest number of eggs about 145.865 from the treatment P3 (AI, oxytocin, ovaprim, PGF2a). Abstrak Pemijahan buatan pada ikan patin dilakukan dengan cara pengurutan karena tidak memiliki kemampuan untuk menge-luarkan telur secara alami. Teknik pengurutan berdampak stres pada induk, kualitas gamet menurun, dan gonad menjadi rusak. Proses pematangan gonad dan pemijahan tanpa pengurutan dapat diinduksi secara hormonal untuk membantu ovulasi ikan yang sulit memijah di luar habitatnya. Penelitian ini bertujuan mengevaluasi penggunaan hormon penghambat aromatase (aromatase inhibitor - AI) dan oksitosin serta kombinasi hormon untuk merangsang ovulasi dan pemijahan pada ikan patin tanpa pengurutan(semi alami). Perlakuan kombinasi hormon terdiri atas P1(AI+Oksitosin), P2 (AI+Oksitosin+Ovaprim), P3 (AI + Oksitosin + Ovaprim + Pgf2a), P4 (ovaprim) sebagai kontrol positif, dan P5 (NaCl) sebagai kontrol negatif. Pada setiap perlakuan digunakan lima induk ikan patin jantan dan lima induk ikan patin betina sebagai ulangan individu dengan bobot berkisar 2-5 kg. Perlakuan diberikan satu kali dengan cara penyuntikan hormon ke dalam jaringan pada bagian otot dibawah sirip punggung (intramuscular). Hasil penelitian menunjukkan perlakuan P3 berhasil menginduksi ikan untuk ovulasi dan memijah tanpa pengurutan, sedangkan pada perlakuan P4 ikan memijah dengan cara pengurutan, dan pada P5 ikan tidak ovulasi dan tidak memijah. Perlakuan kombinasi hormon menyebabkan konsentrasi estradiol-17p dan testosteron plasma menurun yang menunjukkan tahap pematangan akhir. Lama waktu ovulasi tidak berbeda nyata yaitu berkisar antara 12,35±4,05 sampai 15,20±2,25 jam, sedangkan jumlah telur yang diovulasikan tertinggi adalah 145.865butir pada perlakuan kombinasi hormon P3 (AI + Oksitosin + Ovaprim + Pgf2a).
The effectiveness of the papaya seed (Carica papaya L) for reproductive function of Tilapia, Oreochromis niloticus (Linnaeus, 1758) Munawar Khalil; Yunidar Yunidar; Mahdaliana Mahdaliana; Munawwar Khalil; Rachmawati Rusydi; Zulfikar Zulfikar
Jurnal Iktiologi Indonesia Vol 19 No 1 (2019): February 2019
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v19i1.434

Abstract

Introduction fish in Indonesia has been negatively impact to the water ecology, such as declining in the number of native fish species. Handling the negative impacts of introduced fish can be minimized by reducing fertility rates through the use of plant compound extracts. This study was conducted in May - June 2016 which aimed to assess the effectiveness of the papaya seed flour (Carica papaya L) for the reproductive function of introduction fish Oreochromis niloticus. The research design used in this study was completely randomized design, non-factorial with five treatments and three replications, namely A: control (without giving the flour), B: 40 mg, C: 50 mg, D: 60 mg, E: 70 mg of papaya seeds flour mixing in 100 grams of artificial feed. The fish sample used were 45 mature tilapia fish, 4-5 months old with 200 g in weight for female and 250 gram in weight for male. Parameters measured in this research were feed consumption level, fecundity, fertilization rate, hatching rate and sperm morphological structures. The results of this study indicated that the papaya seed flour gave the multiple effect on the sperm abnormality and decreased the motility level of sperm. Statistical analysis showed that the application of papaya seed flour gave significantly different effect (P <0.05) between treatments on percentage of fertilization level and hatching rate parameters, but showed not significantly different on survival rate parameter where P> 0.05. The lowest sperm motility rate was found in treatment E, which was 00.45 ".032 /second. The average eggs number in this study ranged from 994,33 to 1.416 grains, whereas the lowest fertilization level and the lowest hatching rate was in treatment E with the percentage 58.58% and 99.24%. Abstrak Ikan introduksi di Indonesia telah menimbulkan beberapa dampak negatif terhadap ekologi perairan, diantaranya adalah menurunnya spesies ikan asli. Penanganan dampak negatif ikan-ikan introduksi dapat diminimalkan dengan cara menurunkan angka fertilitas melalui penggunaan ekstrak senyawa tumbuhan. Penelitian ini dilaksanakan pada bulan Mei – Juni 2016 yang bertujuan untuk menganalisis efektivitas tepung biji pepaya (Carica papaya L) dalam menurunkan fungsi reproduksi ikan nila Oreochromis niloticus. Rancangan penelitian yang digunakan adalah rancangan acak lengkap non-faktorial dengan lima perlakuan tiga ulangan yaitu A: Kontrol (tanpa pemberian tepung), B: Pemberian 40 mg, C: 50 mg, D: 60 mg, E: 70 mg tepung biji pepaya dalam 100 g pakan buatan. Ikan sampel yang digunakan adalah induk ikan nila sebanyak 45 ekor yang berumur 4-5 bulan dengan berat bobot tubuh 200 g untuk induk betina dan 250 g untuk induk jantan. Parameter yang diukur ialah daya konsumsi pakan, jumlah telur (fekunditas), tingkat pembuahan telur, tingkat penetasan telur, dan morfologi sperma. Hasil penelitian menunjukkan bahwa tepung biji pepaya memberikan efek terhadap abnormalitas sperma dan menurunkan sintasan sperma, dan penurunan tingkat pembuahan telur (fertilitas rendah). Analisis statistik menunjukkan bahwa pemberian tepung biji pepaya memberikan pengaruh yang berbeda nyata (P<0,05) antar perlakuan pada parameter persentase pembuahan dan penetasan telur, namun tidak berbeda nyata terhadap parameter sintasan (P>0,05). Angka motilitas sperma terendah dijumpai pada perlakuan E yaitu 00.45”.032/detik. Jumlah telur pada penelitian ini berkisar rata-rata 994,33-1416 butir dengan nilai pembuahan terendah dan penetasan terendah terdapat pada perlakuan E dengan persentase 58,58% dan 99,24%.
Pengolahan Tiram Menjadi Kaldu dan Saus Tiram Bagi Pemberdayaan Perempuan di Gampong Jawa Lama Kecamatan Banda Sakti Kota Lhokseumawe Prama Hartami; Zulpikar Zulpikar; Mahdaliana Mahdaliana; Boy Haqi; Putri Maulida
JPM: Jurnal Pengabdian Masyarakat Vol. 1 No. 2 (2020): Nopember 2020
Publisher : Forum Kerjasama Pendidikan Tinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lhokseumawe City is one of the coastal areas that has the potential for potential fishery products and is suitable for development. One of the many fishery products found in the coastal areas of the city of Hokseumawe is oysters. Generally, these oyster seekers are women who live around the coastal area of ??the city of Hokseumawe which has become their daily livelihood. The fishery products obtained by the community have not been managed professionally, so that they have not been able to provide maximum benefits to the welfare of the community. Therefore, education and training are needed for the people of Lhokseumawe City, especially women so that they can increase the selling value of these fishery products. This community service activity will increase their knowledge and skills in processing food made from fishery products, namely oysters. Which later the production of this business will have an impact on improving the community's economy, especially women in the Javanese Village of Lhokseumawe City
The effectiveness of ecdysteroid hormones through feed in increasing growth and reproductive performance of mangrove crab (Scylla sp) Mahdaliana Mahdaliana; Salamah Salamah; Muliani Muliani
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 9: No. 1 (April, 2022)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v9i1.6965

Abstract

This study aims to determine the effect of feeding containing ecdysteroid hormones to increase the growth and reproduction of mangrove crabs so as to produce optimal reproduction of mangrove crabs. This research was conducted from August to October 2021 at the Hatchery and Cultivation Technology Laboratory. The method used in this study was a non-factorial completely randomized design (CRD) with 4 treatments and 3 replications, the addition of ecdysteroid hormone 5.50 ml, 7.50 ml, 9.50 ml and 0 ml. The results showed that the addition of ecdysteroid hormones to the feed significantly affected the moulting rate, weight gain, shell length and width and the survival of mud crabs. The best treatment was found in the 5.50 ml treatment with a molting rate of 16 days, weight gain of 12.09 grams, length of 1.84 cm, width of 7.33 and survival rate of 100%. The water quality during the study showed that it was still in a good range or can be tolerated by mangrove crabs, the water quality range was temperature 26.5-27 oC, pH 7.0-8.0, DO 6.3-6.8 mg, and salinity 34 - 35 ppt.Keywords: Ecdysteroids; Growth; Mangrove Crab; Moulting; Reproduction.
Efektivitas tepung hipotalamus sapi dalam pakan buatan terhadap pertumbuhan benih ikan gurami (Osphronemus gouramy) Mahdaliana Mahdaliana; Zulfikar Zulfikar; Iskandar Iskandar
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Aquatic Sciences Journal, Vol. 5: No. 2 (October, 2018)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v5i2.830

Abstract

AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh pakan yang ditambahkan tepung hipotalamus sapi terhadap laju pertumbuhan optimal pada benih Ikan Gurami (Oshpronemus gouramy, L). Penelitian ini diharapkan dapat dijadikan solusi untuk optimalisasi pertumbuhan benih Ikan Gurami (Oshpronemus gouramy) dengan menggunakan pakan yang diberi tambahan tepung hipotalamus sapi. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap Non Faktorial dengan 5 perlakuan dan 3 ulangan, yakni perlakuan A (Kontrol), B (Tepung hipotalamus sapi 0,05% /gr pelet), C (Tepung hipotalamus sapi 0,10% / gr pelet), perlakuan D (Tepung hipotalamus sapi 0,15% / gr pelet) dan perlakuan E (Tepung hipotalamus sapi 0,20%/gr pelet). Parameter yang diamati adalah pertumbuhan berat, pertumbuhan panjang, kelangsungan hidup dan kualitas air. Hasil penelitian menunjukkan bahwa penambahan tepung hipotalamus sapi ke dalam pakan pellet berpengaruh nyata terhadap pertumbuhan panjang dan pertumbuhan bobot benih ikan gurami namun tidak berpengaruh nyata terhadap kelangsungan hidup ikan gurami (Oshpronemus gouramy). Pertambahan panjang tertinggi yaitu pada perlakuan C (0,29 cm) pertambahan bobot tertinggi yaitu pada perlakuan C (0,35 gr) dan kelangsungan hidup tertinggi yaitu terdapat pada perlakuan B dan C mencapai 76,66 %. Nilai kisaran kualitas air adalah suhu berkisar antara 24-26 0C, pH 6,6 - 7,1, DO antara 4,6 - 5,2 mg/L dan amonia berkisar antara 0,001- 0,296 mg/L.Kata kunci: ikan gurami; tepung hipotalamus sapi; kelangsungan hidup; laju pertumbuhanAbstractThis study aims to determine the effect of addition the cow’s hypothalamous flour to comercial feed, in the optimal growth rate in gourami fingerling (Oshpronemus gouramy, L). The benefits of this study are expected to be used as a solution to optimize the growth of gourami fish fingerling (Oshpronemus gouramy, L) by using feed which is supplemented with cow hypothalamic flour. The design used in this study was a Non-Factorial Completely Randomized Design with 5 treatments and 3 replications, namely treatment A (Control), B (Cow hypothalamic flour 0.05% / g pellets), C (Cow hypothalamic flour 0, 10% / g pellets), treatment D (cow hypothalamic flour 0.15% / g pellets) and treatment E (cow hypothalamic flour 0.20% / g pellets). The parameters observed were weight growth, length growth, survival rate and water quality. The results showed that the addition of cow’s hypothalamus flour into pellet feed significantly affected the long growth and growth of gourami fingerling weight but did not significantly influence the survival of gourami (Oshpronemus gouramy, L). The highest length increase is in treatment C (0.29 cm) the highest weight gain is in treatment C (0.35 gr) and the highest survival is found in treatment B and C reaching 76.66%. The range of water quality is the temperature between 24-26 0C, pH 6,6 - 7,1, DO between 4,6 - 5,2 mg / L and ammonia ranging from 0,001 to 0,296 mg/L.Keywords: gourami; cow hypothalamus flour; survival; growth rate
DIVERSIFICATION TECHNOLOGY PROCESSED COK FISH TO IMPROVE THE SKILLS OF HOUSEWIVES IN TUMPOK TEUNGOH VILLAGE, BANDA SAKTI DISTRICT, LHOKSEUMAWE CITY Mahdaliana; Prama Hartami; Salamah
Global Science Society Vol 4 No 1 (2022): Global Science Society (GSS) Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : LPPM dan PM Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/gss.v4i1.4501

Abstract

Desa Tumpok Teungoh terletak di Kecamatan Banda Sakti, Kota Lhokseumawe. Ikan gabus merupakan salah satu jenis ikan lokal yang memiliki kandungan protein yang sangat tinggi. Selain itu, ikan gabus juga merupakan ikan air tawar yang memiliki kandungan nutrisi terbaik yang sangat bermanfaat bagi kesehatan seperti meningkatkan daya tahan tubuh, penyembuhan luka, menjaga keseimbangan cairan sel serta penyembuhan dan pencegahan penyakit. Sebagai sumber protein yang sangat digemari masyarakat luas, ikan ini dapat diolah menjadi berbagai macam produk pangan. Salah satunya hasil diversifikasi olahan ikan gabus adalah bakso dan nugget. Kegiatan pengabdian masyarakat melalui penyuluhan dan pelatihan ini berdampak positif dalam mengatasi permasalahan yang dihadapi masyarakat ibu rumah tangga di Desa Tumpok Teungoh. Hasil survei menunjukkan persentase ibu rumah tangga yang mengetahui teknologi penganekaragaman pengolahan ikan gabus meningkat drastis. Mereka memahami konsep pengolahan ikan gabus, manfaat dan nilai gizi ikan gabus, teknik pengolahan ikan gabus dan jenis pengolahan ikan gabus. kegiatan ini juga sangat mendukung salah satu program pemerintah dalam Program Gemarikan yang digagas oleh Kementerian Kelautan dan Perikanan, yang bertujuan untuk bersama-sama membangun kesadaran gizi secara individu dan kolektif masyarakat agar mereka suka makan ikan, dengan harapan dapat meningkatkan daya tahan tubuh dalam menghadapi pandemi COVID-19. Para peserta sangat antusias dan berharap dengan mengembangkan produk olahan ikan gabus dapat meningkatkan biaya pendapatan.
TEKNITECHNIQUES FOR CULTIVATION OF CATFISH (Clarias. Sp.) AQUAPONIC SYSTEM BUDIKDAMBER TO IMPROVE STUDENT'S CREATIVITY AT SMKK BUDIDAYA IKAN LELE (Clarias. Sp.) SISTEM AKUAPONIK BUDIKDAMBER UNTUK MENINGKATKAN KREATIVITAS SISWA DI SMK NEGERI 6 LHOKSEUMAWE Salamah; Rachmawati Rusydi; Mahdaliana
Global Science Society Vol 4 No 1 (2022): Global Science Society (GSS) Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : LPPM dan PM Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/gss.v4i1.4516

Abstract

The purpose of this activity is to provide an overview of information regarding the cultivation of catfish in the aquaponic system of Budikdamber (bucket cultivation) to increase student creativity and open up business opportunities for students. This training activity is an illustration of how a bucket (budikdamber) can be used to increase student income and open up knowledge for students. Students can carry out cultivation activities with narrow land and lower costs, besides that this aquaponics system is also effective and efficient to be applied in schools. The cultivation method of catfish with the Budikdamber aquaponic system uses probiotics to maintain water quality during maintenance. The frequency of feeding is twice a day, namely in the morning and evening, the amount of feed given is based on the body weight of the fish. After participating in this activity, students can understand and carry out catfish cultivation with the aquaponic system of budikdamber (cultivating in buckets. The result of this service activity is the development of student insight about catfish farming in buckets (budikdamber) with an aquaponic system and also students' skills in processing and caring for catfish and cultivated plants.Furthermore, the development of catfish enlargement cultivation can optimize the use of available land to increase production yields. Keywords: Aquaponics, catfish, budikdamber system
Induksi maturasi gonad ikan nilem (Ostheochilus hasselti) menggunakan hormon OODEV dengan dosis yang berbeda Mahdaliana Mahdaliana; Munawwar Khalil; Nur Aklima
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 4 No 1: Mei 2022
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v4i1.1199

Abstract

Penelitian ini bertujuan untuk mengetahui dosis hormon Oodev yang terbaik untuk mempercepat proses induksi maturasi gonad induk betina ikan nilem (Ostheochilus hasselti). Penelitian ini dilakukan di Laboratorium Fakultas Universitas Malikussaleh, Aceh Utara dari tanggal 18 Agustus sampai 10 September 2020. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL), dengan menggunakan 12 ekor ikan uji dari 4 perlakuan yaitu perlakuan A (0 ml/kg), B (0,3 ml/kg), C (0,5 ml/kg), D (0,7 ml/kg). Hasil uji ANOVA menunjukkan bahwa induksi maturasi gonad pada ikan nilem (Ostheochilus hasselti) menggunakan Oodev dengan dosis perlakuan A (0 mL/kg) menunjukan hasil berbeda sangat nyata dengan perlakuan D (0,7 ml/kg) terhadap tingkat kematangan gonad, indek kematangan gonad, fekunditas, dan diameter telur. Hasil penelitian ini menunjukkan bahwa perlakuan D dengan dosis 0,7 mL/kg menghasilkan nilai terbaik pada indek kematangan gonad (14,59%), fekunditas (39,923 butir), dan diameter telur (0,82 mm).
Fisiologi dan derajat pembuahan sperma ikan patin siam (Pangasianodon hypophthalmus) diencerkan menggunakan air kelapa muda dan gliserol Mahdaliana Mahdaliana; Rachmawati Rusydi; Aminah Aminah
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 4 No 1: Mei 2022
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v4i1.1200

Abstract

Ikan Patin Siam (Pangasianodon hypophthalmus) merupakan ikan air tawar yangbanyak dibudidayakan secara luas di Indonesia. Penelitian ini bertujuan untuk melihat dosis terbaik dan yang paling bagus untuk pengenceran sperma serta mengetahui pengaruh pengenceran sperma menggunakan air kelapa muda dan gliserol terhadap motilitas spermatozoa, kelangsungan hidup spermatozoa, derajat pembuahan telur, derajat penetasan telur serta kualitas air. Penelitian ini dilaksanakan pada Bulan Mei sampai Juni 2018 bertempatdi UPT Budidaya Dinas Pertanian dan Kelautan Kota Medan Tuntungan. Metode penelitian ini menggunakan metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 5 perlakuan dan 3 ulangan yaitu A (kontrol), B (air kelapa muda 60% + gliserol 40%), C (air kelapa muda 50% + gliserol 50%), D (air kelapa muda 40% + gliserol 60%) dan E (air kelapa muda 30% + gliserol 70%). Hasil penelitian menunjukkan bahwa motilitas spermatozoa, derajat pembuahan telur dan derajat penetasan telur tidak bebeda nyata tetapi berpengaruh sangat nyata terhadap kelangsungan hidup spermatozoa. Perlakuan terbaik terdapat pada perlakuan B dengan pengenceran sperma menggunakan air kelapa muda 60% dan gliserol 40%.
Production of Clarias gariepinus seeds through accelerated female broodstock rematuration Prama Hartami; Mahdaliana Mahdaliana; Eva Ayuzar
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.074 KB) | DOI: 10.13170/depik.11.1.22860

Abstract

This study aims to determine the effectiveness of Oodev and Turmeric flour on feed to accelerate the rematuration of female broodstock catfish for increasing seed production. Oodev hormone is believed to accelerate the maturation process of broodstock, while turmeric flour with its active ingredients can improve liver performance in the vitellogenesis process. The method was a non-factorial completely randomized design with 4 treatments and 3 replications. The treatments are Control, (A) Oodev 0.3 ml/kg of feed, (B) Turmeric flour 5 gr/kg of feed, and (C) Combination of Oodev + Turmeric flour. The results showed that C treatment was the best treatment which produced a GSI value of 14.57 ±2.53%, period of rematuration was 32.33 ±4.16 days, egg diameter was 1.27 ±0.12 mm, fecundity was 22,939.97 ±880.38 eggs/kg of broodstock weight, fertilization rate was 80.18 ±9.75%, hatching rate was 79.80 ±2.88%, and larval survival reached 83.17 ±9.69%. Meanwhile, the specific growth of seed length in all treatments had no significant effect between treatments. The results of this study will certainly provide useful information for increasing catfish seed production both in Aceh and on a Nationally. This acceleration of maturation also has a positive impact in maximizing the available broodstock without having to increase the number of catfish broodstock to produce seeds of superior quality and benefit the cultivators.Keywords:EnrichmentFemale BroodstockPerformanceRematurationReproduction