Jurnal Biologi Tropis
Vol. 25 No. 4 (2025): Oktober-Desember

In Vitro Antifungal Activity of Streptomyces and Bacillus Isolates against Fusarium oxysporum f.sp. cubense

Nurhidayati, Febry (Unknown)
Wisanggeni, Gen Adi (Unknown)



Article Info

Publish Date
01 Oct 2025

Abstract

Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense (Foc) represents a major constraint to banana production, leading to severe yield and quality losses. The use of biological control agents (BCAs) such as Streptomyces and Bacillus offers an environmentally sustainable alternative to manage this pathogen. This study aimed to evaluate the antagonistic activity, culture filtrate effects, volatile organic compounds, and compatibility of Streptomyces S01, Bacillus B01, and Bacillus B16 isolates against Foc under in vitro conditions. A completely randomized design (CRD) with three replications was employed. Results indicated that Streptomyces S01 was the most effective isolate, achieving 84.12% inhibition in the dual culture assay, 69.96% in the culture filtrate test, and 41.4% through volatile compound activity. Isolates Bacillus B16 and Bacillus B01 showed moderate efficacy, with Bacillus B16 exhibiting superior performance in the volatile assay (28.8%). Compatibility tests revealed that Streptomyces S01 + Bacillus B16 and Bacillus B01 + Bacillus B16 were mutually compatible, while Streptomyces S01 + Bacillus B01 displayed incompatibility. These findings suggest that Streptomyces S01, particularly in combination with Bacillus B16, holds strong potential as a microbial consortium for the biological control of Fusarium wilt in banana cultivation.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...