Jurnal Riset Kimia
Vol. 16 No. 2 (2025): September

Classification using FTIR and UV-Vis spectra combined with chemometrics and GC-MS profiles of Brucea javanica fruit extracts with different extracting solvents

Triawan, Deni Agus (Unknown)
Banon, Charles (Unknown)
Nurwidiyani, Ria (Unknown)
Adfa, Morina (Unknown)



Article Info

Publish Date
30 Sep 2025

Abstract

Brucea javanica L. Merr. has long been used for its antimalarial, anti-diabetic, antibacterial, and antioxidant effects. Its bioactivity depends on both its chemical constituents and the type of solvent employed during extraction. Integrating FTIR and UV-Vis spectral data with chemometric approaches enables classification of fruit extracts based on the solvent used, while GC-MS analysis provides detailed compound identification. Using sonication, extraction with methanol, ethyl acetate, and n-hexane produced yields of 30.61% ± 0.80, 19.20% ± 0.75, and 18.12% ± 0.89, respectively. FTIR spectra were recorded over 600–4000 cm⁻¹, and UV-Vis spectra were measured across 200–800 nm. Analysis showed that ethyl acetate and n-hexane extracts exhibited similar profiles, which differed from that of the methanol extract. PCA successfully distinguished all three extracts, with cumulative PC1 and PC2 values above 70%. FTIR-based PCA provided better separation due to its broader fingerprint region compared to UV-Vis spectra. GC-MS results revealed that the compound profiles were largely similar across the extracts, although more compounds were detected in the methanol extract than in the ethyl acetate or n-hexane extracts.

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