Storage procedures are the main factor that influences the quality of platelet concentrate. In vitro, platelet concentrate is stored in a platelet agitator at a temperature of 20ºC-24ºC for 5 days. This study aims to determine the difference in the number of platelets in platelet concentrate with and without the agitation process. This type of research is quasi-experimental using a time series design with an independent t-test. The research was conducted in April 2024. Samples of 16 bags of platelet concentrate with and without the agitation process which were stored on the 0th, 1st, 2nd, 3rd, 4th, and 5th days were checked for platelet counts. In the normality test of the platelet examination data, the p-value was <0.05, so it was stated that the data was not normally distributed so it had to be continued with the Mann-Whitney U test. The research results showed that the p-value on days 0, 1, 2, and 3 was >0.05, indicating no significant difference in the number of platelets between the groups with and without the agitation process. Meanwhile, on days 4 and 5, a p-value <0.05 was found, and there was a difference in the number of platelets with and without the agitation process. It can be concluded that agitation plays an important role in maintaining platelet stability after the third day of storage, indicating that the agitation process is necessary to preserve platelet quality during longer storage periods.
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