Cytotoxic Activity of Pentacyclic Triterpene-3-Heptadecanoate Ester against Hela Cell Line and Its Docking Study

Sri Mulyani Mulyadi (Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta)
Tri Yuliati
Maulana Tegar
Imam Ardhila

MPI (Media Pharmaceutica Indonesiana)
Vol. 1 No. 1 (2016): JUNE

Pentacyclic triterpene-3-heptadecanoate (12,13-dihydro-α-amyrin-20,30-en-3-heptadecanoate)ester refers to the compound as a result of reaction between pentacyclic triterpene-3-ol(12,13-dihydro-α-amyrin-20,30-en-3-ol) and heptadecanoic acid. This research was aimed to conductcytotoxicity test of pentacyclic triterpene-3-heptadecanoate; 12,13-dihydro-α-amyrin-20,30-en-3-oland 12,13-dihydro-α-amyrin-20,30-en-3-acetate esters against Hela cell line. The activity assay wascarried out using MTT method. The results indicated that IC50 value of 12,13-dihydro-α-amyrin-3-heptadecanoate; 12,13-dihydro-α-amyrin-20,30-en-3-acetate and 12,13-dihydro-α-amyrin-20,30-en-3-ol were 111.0, 151.1 and 944.4 μg/ml, respectively. The compound of 12,13-dihydro-α-amyrin-20,30-en-3-heptadecanoate had the lowest IC50 value, suggesting it has a potency to be synthesized as ananticancer drug.