<![CDATA[Emissions of ammonia gas (NH3) produced from the manure of laying hens can harm health and productivity. This study aims to determine the comfort level of laying hens based on the temperature humidity index which is calculated based on the temperature and humidity conditions of the cage, and its relationship with ammonia gas (NH3) levels, as well as the factors that most influence the concentration of ammonia gas (NH3) around the cage. Data collection was carried out in 96 units of laying hen cages in Sidrap Regency spread across 4 sub-districts, including; Kulo, Pitu Riawa, Maritengngae, and Watang Pulu. Data collection was carried out directly by measuring the ammonia levels in the cage air using the Ammonia Gas Detector Smart Sensor AR8500 system. Temperature and humidity were measured using an Elitech DT-3 Thermo Hygrometer. Data were analyzed by correlation and multiple linear regression. The results of the research showed that as many as 53.13% of laying hens were in an "uncomfortable" condition in breeder-rearing cages in Sidrap Regency. Air ammonia (NH3) levels have a "strong" correlation with humidity and a "moderate" correlation with THI. The higher the humidity and THI, the higher the ammonia (NH3) levels in the air. Higher humidity will increase the ammonia (NH3) content in the cage air partially and simultaneously with the regression equation. Air NH3 level = -11.803 + 1.328 temperature + 0.152 humidity – 1.314 THI.]]>
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