Jurnal Ilmu Pertanian Indonesia
Vol. 0 No. 00 (2025): inpress

SELEKSI DAN IDENTIFIKASI Bacillus HIDROLITIK DARI PENGOMPOSAN SERASAH: SELECTION AND IDENTIFICATION OF HYDROLYTIC BACILLUS FROM LITTER COMPOSTING

Mubarik, Nisa Rachmania (Unknown)



Article Info

Publish Date
10 Oct 2025

Abstract

Potential waste-degrading microbes have long been the focus of research in efforts to increase the efficiency and effectiveness of waste processing, one of which is the Bacillus group. This research aims to select and identify Bacillus sp from composting litter. The samples used were litter from composting with five different treatments, two of which were successive additions of nutrient broth (NB) and formula, while the other three treatments were molasses, tryptic soy broth (TSB), and talc which were added by the consortium. Isolation was carried out using the serial dilution method, followed by purification, and tested for hemolysis potential on blood agar media, while the cellulolytic, amylolytic and proteolytic abilities were carried out by growing the isolate on solid agar media carboxyl methyl cellulose (CMC), starch and skim. Isolates that had three abilities based on the hydrolysis index were tested as antagonists. Isolates that were not mutually antagonistic were identified based on phenotype (colony morphology and Gram staining) and genotype (16S rRNA gene). Fourteen isolates suspected to be Bacillus sp were known to have cellulolytic, amylolytic and proteolytic abilities. Four isolates were selected, two of which had similarity to Bacillus stercoris, while the other two isolates, namely 5.5 and 5.9R, each had similarity to Calidifontibacillus erzurumensis and Bacillus amyloliquefaciens. Litter waste can be a source of searching for potentially hydrolytic Bacillus isolates. Keywords: amylolytic, decomposer bacteria, cellulolytic, proteolytic

Copyrights © 2025






Journal Info

Abbrev

JIPI

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Artikel yang dimuat meliputi hasil-hasil penelitian, analisis kebijakan, dan opini-opini yang berhubungan dengan pertanian dalam arti luas, seperti agronomi, ilmu tanah, hama dan penyakit tanamam, ilmu kehewanan, kedokteran veteriner, keteknikan pertanian, teknologi industri, teknologi pangan, ilmu ...