<![CDATA[This study aims to determine the chemical quality and fermentability of the rumen contents of female Bali Timor cattle in vitro. This research used an experimental method with T-test analysis (comparative test). The number of samples for each was 15 female Timor Bali cattle as replicates, taken from two different slaughterhouses, namely Bimoku abattoir (government-owned) and Aldia Oben abattoir (privately owned). The variables observed were crude protein, crude fiber content, and concentrations of VFA and NH3. The data obtained were analyzed using a T-test (independent sample t-test) at a significance level of 5%, namely comparing the quality of the cattle rumen contents of Bimoku and Oben abbatoir. All data were processed with SPSS 25 software. The statistical analysis results of the t-test showed that the crude protein content and concentration of NH3 of the two locations was not significantly different (P> 0.05), the crude fiber content of Bimoku RPH was significantly higher (P<0.05) than RPH Oben, and VFA concentration from RPH Oben was significantly (P<0.05) higher than Bimoku RPH. It was concluded that differences in abattoir management before slaughtering affected the quality of the rumen contents of female Timor Bali cattle]]>
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