Optimization of Saccharomyces cerevisiae Dose in Eco-Enzyme Fermentation: Effects on pH, BOD, DO, Nitrite, and Nitrate

Dewi, Endah Rita Sulistya
Ulfah, Maria
Nugroho, Ary Susatyo

Advance Sustainable Science, Engineering and Technology (ASSET)
Vol. 7 No. 4 (2025): August-October

The contribution of Saccharomyces cerevisiae is expected to accelerate the fermentation process and enhance the microbial population involved in eco-enzyme production. This study aimed to analyze the potential role of S.cerevisiae in improving eco-enzyme production. A true experimental method was employed using a completely randomized design with four treatments and three replications. The results, based on the average matrix similarity test using the Wilks’ Lambda Test, showed that the significance values for pH, DO, BOD, nitrite, and nitrate were < 0.05. Therefore, H0 was rejected, indicating that varying doses of S.cerevisiae had a significant effect. Furthermore, significant differences were observed among the treatments for each dependent variable. In conclusion, S.cerevisiae contributes to enhancing the overall quality of eco-enzymes by facilitating fermentation and accelerating the decomposition of organic matter.