MASALIQ: Jurnal Pendidikan dan Sains
Vol 5 No 5 (2025): SEPTEMBER

Analisis Kadar Vitamin A pada Minyak Goreng Sawit dengan Metode HPLC (High Performance Liquid Chromatography)

Asaumi, Suci (Unknown)
Azhar, Minda (Unknown)



Article Info

Publish Date
10 Aug 2025

Abstract

This study is motivated by the importance of vitamin A content in palm cooking oil as an effort to prevent vitamin A deficiency in the community. The objective is to measure the vitamin A content in palm cooking oil using the High Performance Liquid Chromatography (HPLC) method in accordance with SNI 7709-2019. Samples of palm cooking oil were obtained from a store on Jl. Sail, with the analytical procedure involving the preparation of standard vitamin A solutions in acetone and ethanol, the addition of pyrogallol acid as an antioxidant, saponification with 50% KOH, and the addition of glacial acetic acid to improve extraction efficiency. HPLC analysis was conducted using a mobile phase of methanol–aquabidest (860:140) and THF–ethanol (50:50) as the solvent. The chromatogram showed a vitamin A peak at a retention time of approximately 11.40 minutes. The calculated results indicated an average vitamin A content of 1,095.32 IU/g, far exceeding the minimum limit of 45 IU/g as required by the quality standard for palm cooking oil. The study concludes that the HPLC method with this procedure can accurately measure vitamin A content, and the tested palm cooking oil samples meet the vitamin A standard for public consumption.

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Journal Info

Abbrev

masaliq

Publisher

Subject

Religion Education Social Sciences

Description

MASALIQ: Jurnal Pendidikan dan Sains was published by Lembaga Yasin alSys which was published six times a year, January, March, May, July, September and November with a minimum of 5 articles. The journal aims to provide a forum for scholarly understanding of the field of education and plays an ...