<![CDATA[Sembilang is one of the fish that is abundant in Merauke mainland waters. Genetic research needs to be done to prevent the decline of catfish population. DNA barcoding is a taxonomic method that uses short genetic markers in an organism's DNA to identify species. This research aims to identify the Sembilang fish species using the Cytochrome Oxidase I gene. This study is an exploratory survey research. Sample collection was carried out in the waters of Padi Swamp, Merauke Regency. The mitochondrial cytochrome oxidase I (COI) gene was amplified using the Polymerase Chain Reaction (PCR) method with FISH-BCL and FISH-BCH primers. The results of analysis using DNA barcoding revealed that the samples collected were the Sembilang species (Neosilurus ater) with a DNA sequence length of 660 bp. Phylogenetic reconstruction using the Neighbor-Joining method with the 2-Parameter Kimura Model shows sequence grouping based on similarity and genetic distance between species.]]>
Copyrights © 2024
