Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation

Shanti Ratnakomala (Major Microbiology, Graduate School Bogor Agricultural University (IPB) and Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI))
Yopi Yopi (Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI))
Maggy Thenawidjaja Suhartono (Faculty of Agricultural Engineering and Technology, Bogor Agricultural University (IPB))
Anja Meryandini (Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University (IPB))
Bambang Prasetya (National Standardization Agency of Indonesia (BSN))

Annales Bogorienses
Vol. 18 No. 2 (2014): Annales Bogorienses

The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml), (NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28C for 2 days incubation.