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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
Core Subject :
Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 189 Documents
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ANTI-DIABETIC EFFECT OF 70% ETHANOL EXTRACT OF Anredera cordifolia (Teen) Stennis (BINAHONG) LEAVES ON GLUCOSE TRANSPORTER 4 (GLUT4) AND PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA (PPAR-γ) GENES EXPRESSION IN 3T3-L1 ADIPOCYTE CELLS Asri Sulfianti
Annales Bogorienses Vol. 25 No. 1 (2021)
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The evidence of Anredera cordifolia (Binahong) as anti-diabetic at molecular stage has not been elucidated. Present study, we investigate the antidiabetic potential of Binahong by evaluating its effect on two diabetic gene markers expression, Translocation of Glucose Transporter 4 (GLUT4) and Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) in 3T3-L1 adipocytes. The investigation has been done by semi quantitative real time PCR. Our finding shown, the GLUT4 MRNA levels were dose dependent excess in adipose treated by 6.25-12.5 mg/ml of 70% Binahong ethanol extract. Though, at 50 mg/ml dose, the GLUT4 expression was up down to 1.2 fold. In addition, the pattern of PPAR-γ MRNA levels in 3T3-L1 were almost same. Every Binahong extracts gave increment on PPAR-γ MRNA levels except 3.125, 6.25, and 50 mg/ml doses. In undifferentiated adipocytes, GLUT4 and PPAR-γ mRNA were barely expressed. The up regulation of those MRNA levels probably occurred because of Binahong has an active compound. However, it is not yet known which compounds who regulate the expression and induces translocation of GLUT4. We hypothesis that it acts as ligand for the PPAR-γ transcriptional factor which activates the GLUT4 transcription and plays orchestra in glucose homeostasis at molecular level.
COMPARATIVE ANALYSIS OF VIRULENCE FACTOR BETWEEN DIFFUSIBLE SIGNAL FACTOR BIOSENSOR AND INDONESIAN Xanthomonas oryzae pv oryzae Fatimah; Iqbal Kusnandarsyah; Apon Zaenal Mustopa; Nurlaili Ekawati
Annales Bogorienses Vol. 25 No. 1 (2021)
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Xanthomonas oryzae pv. Oryzae (Xoo) is the causal agent of bacterial blight in rice (Oryza sativa L.). In the understanding of virulence determinants of three Indonesian Xoo, extracellular polysaccharide (EPS) production, cellulose and diffusible signal factor (DSF) were identified. DSF signaling system in Xoo is involved in the regulation of virulence factor production. In this study the comparative analysis was conducted with the DSF biosensor isolate (Xoo-dsf). The results showed that high yield of EPS and cellulose activity revealed from isolate Xoo1130 and Xoo1122 compare to Xoo93229 while Xoo-dsf giving similar result with Xoo1130. The comparative analysis of Xoo1130 and Xoo-dsf showed that Xoo1130 produced three DSF-family signals (DSF, BDSF and CDSF) similar to the DSF biosensor isolate. It assumed that Xoo1130 uses a similar mechanism in Xoo-dsf to autoregulate the biosynthesis of the DSF-family signals.
Fermentation Tofu Using Consortium Lactic Acid Bacteria Isolated from Whey Tofu as Biocoagulant and Bioperservation Siti Eka Yulianti; Dea Indriani Astuti
Annales Bogorienses Vol. 25 No. 1 (2021)
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Tofu is a traditional food that has high protein content and is prone to spoilage. A fermentation process with consortium lactic acid bacteria (LAB) that can produce bacteriocin has is carried out to get tofu that is more durable with better taste. The Bacteria which are used in this study are Lactobacillus fermentum, Leuconostoc mesenteroides, and Lactobacillus plantarum. L. fermentum is isolated from whey tofu. The antibacterial ability of each bacteria is measured by bacteriocins assay after incubation at 0 hours; 4 hours; and 8 hours in a row are L. fermentum 0; 2506.429 AU/mL; 1502.679 AU/mL; L. mesenteroides 0 AU/mL; 2506.43 AU/mL; 2939.44 AU/mL; L. plantarum 760.39 AU/mL; 3341.25 AU/mL; 3889.29 AU/mL. Ratio inoculum bacteria (a:b:c) which are used are 2:1:1, 1:1:2, and 1:1:1 by using percentage of inoculum 10%. The Optimum inoculum age of these bacterium is 6 hours with µmax each bacteria in a row is 0.04 h-1, 0.02 h-1, dan 0.02 h-1. The fermentation is processed in 8 hours at 37ᵒC of temperature without agitation. From this optimation, the highest bacteriocin activity in tofu curd is 1767.857 AU/mL, which is in a ratio of 1:1:2 at the 4th hour.
Acid-producing Bacteria and Yeasts Isolated from Arabica Coffee Plantations in Central Java and East Java, Indonesia Dinihari Indah Kusumawati; Toto Iswanto; Puspita Lisdiyanti
Annales Bogorienses Vol. 25 No. 1 (2021)
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The converting process of coffee cherries to green coffee beans was conducted through the fermentation process using lactic acid bacteria (LAB) and yeasts to degrade coffee's mucilage. This present study aims to isolate acid producing bacteria and yeasts from three type of samples obtained from Arabica Coffee Plantations in Central Java and East Java, Indonesia. A total of 31 bacteria and 15 yeasts were obtained from this study. From 31 bacteria, 6 strains were identified as Lactobacillus sp. isolated from all samples; 15 strains were identified as P. pentosaceus; 4 strains as L. pseudomesenteroides; 1 strain as K. quasipneumoniae; and 5 strains as A. tropicalis from exocarp of coffee fruit. While from 15 yeast, 8 strains were identified as C. tropicalis isolated from soil; 2 strains as P. kudriavzevii from Luwak feces, and 5 strains as C. fabianii from exocarp of coffee fruit. A total of 25 strains of lactic acid bacteria can release lactic acid bacteria on MRS agar supplemented with 0.5% CaCO3, but 1 strain of K. quasipneumoniae and 5 strains of A. tropicalis produced acid weakly. Four strains of C. tropicalis showed the ability to form thin clear zone formation contrariwise with four other strains. This study adduced that waste materials and soil from the plantation coffee’s area are potential sources to obtaining acid-producing bacteria which is utilized in fermentation process of coffee cherries.
POTENTIAL OF GROWTH HORMONES IAA OF ENDOPHITIC MICROBES FROM NEEM PLANTS FOR CAPITAL OF INDUSTRIAL OPPORTUNITY Sylvia J R Lekatompessy; Nuriyanah; Liseu Nurjanah; Eleazar Handoyo; Rumella Simarmata; Tiwit Widowati
Annales Bogorienses Vol. 25 No. 1 (2021)
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Indonesia has a tropical climate that is rich in diversity of microorganisms with useful potential and has important values that can be developed in the fields of industry, health and agriculture. Neem is a plant commonly used as an insecticide for pest control. Biotechnology continues to develop in exploiting the unique and interesting potential of endophytic microbes to obtain useful bioactive compounds extracted from plants, without having to take large amounts of plant biomass. This study aims to explore other potentials of endophytic microbes from neem plants, namely as a producer of IAA growth hormone to support plant growth. The results of the test of growth hormone-producing microbes, from 19 isolates of endophytic bacteria tested, obtained 3 isolates of IAA hormone-producing bacteria. 36 isolates of endophytic fungi were tested, 18 isolates of endophytic fungi were positive for IAA hormone production. One of the isolates of endophytic fungi that produces the highest IAA hormone, has been identified molecularly and the result is Colletotrichum gloeosporioides. The potential value derived from the endophytic microbes of neem plants can be developed into biotechnology products that have high commercial value by utilizing potential genes to support plant growth.
Synthesis and Characterization of Alginate-Cellulose Xanthate Beads from Corn Stalk with NaCl As Porogen Rohmatullah, Wahyu Adhi Putra; Yulianti, Eny; Khoiroh, Lilik Miftahul; Mahmudah, Rif'atul
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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In this study, the synthesis of porous beads from corn stalks was carried out. The cellulose extracted from corn stalks was converted into cellulose xanthate and combined with alginate to form porous alginate-cellulose xanthate beads by the ionic gelation method. This study attempted to use sodium chloride (NaCl) as a porogen and zinc acetate as a crosslinker. Beads were characterized to determine the porosity, swelling properties, and functional groups using Fourier Transform Infra-Red (FTIR). The geometry of beads was analyzed by optical microscopy, and its surface morphology was analyzed by Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX). The results showed that corn stalks as agricultural waste material could be used to synthesize porous beads material. The swelling and porosity of beads increased with increasing concentration of NaCl. The presence of porogen has increased beads formation. The results demonstrate the crosslinks between zinc acetate and alginate were successfully characterized using FTIR. NaCl concentration of 9.5% resulted in the highest swelling properties (52.80%) and porosity (81.4%) of the beads.
Study on Endophytic Fungi Associated with Moringa oleifera Lam. Collected from Lombok Island, West Nusa Tenggara Ramadhani, Indriati; Rohadi, Hasnadiazahra; Yuliani, Yeni; Ilyas, Muhammad
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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An assemblage of endophytic fungi was isolated from Moringa oleifera Lam. collected from Lombok island, West Nusa Tenggara Province, Indonesia. Fungal endophytes were isolated using surface sterilization methods with slight modification. Forty-six selected endophytic fungal strains were isolated from the leaves, petioles, and stems of M. oleifera. The fungal strains identification through morphological observation and ITS rDNA-based molecular analysis showed that fungal endophytes were associated with host plants belonging to the taxa Alternaria, Cladosporium, Colletotrichum (Glomerella), Corynespora, Curvularia (Cochliobolus), Fusarium, Mucor, Ochrocladosporium, Phomopsis (Diaporthe), and Trametes. In this study, endophytic Phomopsis dominating the obtained strains, whereas 26% (12/46) strains were isolated from host plant samples.
The Purification of Rennin-Like Protease from Lactobacillus paracasei Isolated from Ettawa Goat Milk Putranto, Wendry Setiyadi; Mustopa, Apon Zaenal; Kusumawati, Arizah; Prastyowati, Anika
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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There is a protease produced by bateria that has characteristics similar to rennin from a calf. Rennin has the ability to clot casein in milk. Rennin-like protease (RLP) is produced by bacteria extracellularly. Lactic Acid Bacteria (LAB) have the potential to be developed for RLP production because they are safe and non-pathogenic bacteria. Rennin is needed in the process of milk coagulation to subsequently obtain a curd in the process of making cheese. In this study, the LAB isolated from Ettawa goat milk (isolate 2.12) which produced RLP was 99% identical to Lactobacillus paracasei based on 16S rRNA gene sequence analysis. The purification of the RLP L. paracasei 2.12 with 60% ammonium sulfate deposition, dialysis, and filtration gel chromatography Sephadex G-50 showed a single 38 kDa protein band with SMCA/SPA was 4.48 higher than that of the calf rennet with a ratio value of 1, therefore in this study, RLP L. paracasei 2.12 was developed as an alternative to renin in cheese making.
Inhibitory Potential of Black Seed (Nigella sativa L.) Bioactive Compounds Towards Main Protease of SARS-CoV-2: In Silico Study Ferdian, Pamungkas Rizki; Elfirta, Rizki Rabeca; Emilia, Qori; Ikhwani, Azra Zahrah Nadhirah
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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COVID-19, caused by SARS-CoV-2, has become a massive worldwide concern of the 21st century. One potential strategy to block the biochemical pathway of SARS-CoV-2 was by inhibiting the main protease (Mpro), which is a key enzyme on viral replication. Black seed (Nigella sativa L.) has a long history for its use as a traditional medicine. Therefore, we hypothesised that the black seed contains numerous active compounds that could potentially confer inhibitory activity against SARS-CoV-2 viral Mpro. In this study, 24 active compounds from black seed were tested. Compounds were screened using Lipinski's Rules and admetSAR, then docked to viral Mpro 7BQY by AutoDockTools-1.5.6 and AutoDock Vina using a site directed docking approach resulting in affinity energy (∆G) and binding data. We found that the most potential active compound of N. sativa is 3-[(4-Methylphenyl)sulfanyl]-1,3-diphenyl-1-propanone, since its affinity energy was -7.6 kCal.mol-1. Its similarity to N3 inhibitor based on Ligplot analysis and DS were 86.7% and 76.19%, respectively, and the occupancy on binding site based on Ligplot analysis and DS were 90.91% and 81.82%, respectively. These findings can be used as a starting point for further investigation using in vitro and in vivo studies.
Enhancement of Yield, Starch, and Amylose Content of Two Indonesian Cassava Genotypes by Producing Gamma Irradiated-Induced Mutants Sudarmonowati, Enny; Hartati, N. Sri; Supatmi, Supatmi
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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As an important carbohydrate-source food crop and raw materials of starch-based industries, accelerated cassava breeding has been extensively pursued through various procedures, among others, is mutant induction using gamma irradiation combined with molecular marker. Iding, a local genotype cassava has been compared with a national variety Adira 4 for its yield attributes following gamma irradiation at various dose levels ( 2, 20, 30, and 50 krad). The mutation rate was conducted based on morphological characteristics and identification using RAPD by employing random primer OPB-10. Evaluation was conducted on mutant lines producing high potential yield, high starch and amylose contents. The fourth generation of irradiated Adira 4 (2 and 30 krad) and Iding (2 krad) exhibited higher yield and amylose content as compared to their representative controls. The yield of 30 krad irradiated Adira 4 indicated by mean tuber weight per individual could reach more than twice of that of control and almost 1.5 times of that irradiated with 2 krad. The levels of amylose content of all irradiated ones, regardless of genotypes and gamma irradiation doses (2 and 30 krad) were higher than that of control. In most parameters, irradiated Adira 4 was superior to irradiated Iding.

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