JURNAL PEMULIAAN TANAMAN HUTAN
Vol 5, No 1 (2011): Jurnal Pemuliaan Tanaman Hutan

FRAGMEN POLIMORFIK PENANDA RAPD UNTUK ANALISIS GENETIK SENGON (Falcataria moluccana)

Vivi Yuskianti (Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan)



Article Info

Publish Date
15 Jul 2011

Abstract

This research was conducted to support tree improvement program for sengon (Falcataria moluccana (Miq.) Barneby & J.W. Grimes). Polymorphic fragments were surveyed in 288 RAPD primers using 24 materials from Okinawa, Japan. Carefully selections of the polymorphic fragments were applied to overcome a problem of low reproducibility of RAPD markers. The first screening using 4 materials found that 285 out of 288 primers successfully produced fragments. The second screening using 16 materials finally obtained 48 polymorphic fragments. High discrimination ability (DP=0.483, in average) was obtained from all the selected fragments. The selected fragments can be used to support tree improvement program of the species for genetic diversity study and to facilitate a development of more specific markers such as Sequence Characterized Amplified Region (SCAR) marker.

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