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All Journal JURNAL PEMULIAAN TANAMAN HUTAN Jurnal Penelitian Kehutanan Wallacea
Vivi Yuskianti
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan
Agriculture, Biological Sciences & Forestry

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IDENTIFIKASI KLON JATI (Tectona grandis Linn F.) MENGGUNAKAN PENANDA SCAR (Seguence Characterized Amplified Region). Vivi Yuskianti
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 3 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2009.3.3.139-146

Abstract

An easy, Cheap and acacurate identification method is needed to ensure the identity of teak clones used in planation. Molecular markers offer an alternative technique for clonal identivication . one such method is SCAR markers. Seventy teak clones of which 20 clones were produced by tissue cultures and claimed to be originated from the some parent. And 50 clones from buddings were used as materials. Analysis was conducted using 6 highly polymorphic SCAR primers. The results showed that 20 teakclones Originated from tissue cultures clustered into 3 groups, Whereas the rest 50 samples from buddings clustered into 4 groups. SCAR markers were able to differentiate the identity of these clones.These SCAR markers can applied to prove the claim abaout the identity of chones in the market and to ovoid labeling errors in the field  for Vegetatively propagated plants.
FRAGMEN POLIMORFIK PENANDA RAPD UNTUK ANALISIS GENETIK SENGON (Falcataria moluccana) Vivi Yuskianti
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 1 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.1.55-61

Abstract

This research was conducted to support tree improvement program for sengon (Falcataria moluccana (Miq.) Barneby & J.W. Grimes). Polymorphic fragments were surveyed in 288 RAPD primers using 24 materials from Okinawa, Japan. Carefully selections of the polymorphic fragments were applied to overcome a problem of low reproducibility of RAPD markers. The first screening using 4 materials found that 285 out of 288 primers successfully produced fragments. The second screening using 16 materials finally obtained 48 polymorphic fragments. High discrimination ability (DP=0.483, in average) was obtained from all the selected fragments. The selected fragments can be used to support tree improvement program of the species for genetic diversity study and to facilitate a development of more specific markers such as Sequence Characterized Amplified Region (SCAR) marker.
Keragaman Genetik Tetua Dan Anakan Dari Kebun Benih Semai Acacia Mangium Grup D (Am004) Di Sumatera Selatan, Indonesia Vivi Yuskianti
Jurnal Penelitian Kehutanan Wallacea Vol. 3 No. 2 (2014)
Publisher : Foresty Faculty of Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.727 KB) | DOI: 10.18330/jwallacea.2014.vol3iss2pp129-137

Abstract

Informasi keragaman genetik dari satu generasi ke generasi selanjutnya merupakan faktor penting untuk pengelolaan dan konservasi genetik kebun benih. Tujuan dari penelitian ini adalah untuk mengevaluasi tingkat keragaman genetik tetua sebagai generasi pertama (F1) dan anakannya (F2) dari kebun benih Akasia mangium yang ada di kebun benih semai Grup D (AM004) di Sumatera Selatan. Analisis dilakukan terhadap 251 tetua dan ± 200 biji dari 10 pohon induk. Analisis menggunakan 12 penanda mikrosatelit menunjukkan bahwa rata-rata jumlah alel yang terdeteksi (A) dari semua tetua dan anakannya adalah 8,23 dan 7.08. Secara umum, tingkat keragaman genetik tetua dan anakan tidak berbeda (He=0.609 untuk tetua dan He=0.606 untuk anakan). Penelitian ini mendeteksi keberadaan alel baru pada anakan. Kondisi ini menunjukkan kemungkinan adanya kontaminasi serbuk sari dari luar kebun benih.
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