<![CDATA[Arctium lappa L. (burdock) root contains phytochemicals—including flavonoids, saponins, and phenolic compounds—reported to exhibit immunomodulatory activity. This study evaluated the safety of Arctium lappa L. root extract through a 28-day post-test experimental design involving male and female Wistar rats. Eight animals were randomized into four groups receiving oral doses of 0, 150, 300, or 450 mg/kg BW/day. Lymphocyte counts were measured using an automated analyzer, and splenic histopathology was assessed on Hematoxylin–Eosin–stained sections using semiquantitative scoring. Lymphocyte values (mean ± SD) showed no significant differences among groups (p > 0.05; small effect size) and remained within physiological limits, with males ranging from 4.14 ± 0.21 to 7.96 ± 0.34 ×10³/µL and females from 2.04 ± 0.18 to 15.43 ± 0.52 ×10³/µL. No necrotic lesions were identified in any dose group. Splenic congestion and extramedullary hematopoiesis were mild to moderate, remaining below 30–50% involvement without architectural disruption. These findings indicated that repeated oral exposure to A. lappa extract up to 450 mg/kg BW/day did not induce structural injury or suppress immune-cell profiles, supporting its preliminary safety for further toxicological and immunopharmacological investigation.]]>
Copyrights © 2025
