<![CDATA[Phanera semibifida Roxb. var. semibifida, commonly known as the butterfly root plant, is one of the plants frequently used by various indigenous tribes in Kalimantan as a traditional medicinal plant. This study was conducted to identify the secondary metabolite compounds, toxicity level, and anti-inflammatory activity of the plant. Extraction of the stem of the butterfly root plant was carried out using the maceration method with methanol as the solvent, followed by partitioning with n-hexane and ethyl acetate. Phytochemical screening of the stem was performed using Thin Layer Chromatography (TLC). Toxicity testing was conducted using the Brine Shrimp Lethality Test (BSLT), and anti-inflammatory activity was evaluated based on protein denaturation inhibition in vitro using a ultraviolet-visible (UV-Vis) spectrophotometer. The crude extract of the stem was found to contain alkaloids, triterpenoids, steroids, flavonoids, and phenolics; the n-hexane fraction contained triterpenoids and steroids; the ethyl acetate fraction contained alkaloids, triterpenoids, steroids, flavonoids, and phenolics, while the aqueous fraction contained alkaloids, flavonoids, and phenolics. The toxicity levels (LC₅₀) of the crude extract, n-hexane fraction, ethyl acetate fraction, and aqueous fraction were 57.45; 158.04; 42.34; and 72.88 µg/mL, respectively, indicating toxic activity against Artemia salina L. larvae. The anti-inflammatory activity levels (IC₅₀) of the crude extract, n-hexane fraction, ethyl acetate fraction, and aqueous fraction were 124.95 µg/mL (moderate category); 161.27 µg/mL (moderate category); 99.15 µg/mL (strong category); and 143.44 µg/mL (moderate category), respectively. These results indicate that the stem of the butterfly root plant (Phanera semibifida Roxb. var. semibifida) contains bioactive compounds and has potential as a natural anti-inflammatory agent.]]>
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